Difference between revisions of "Part:BBa K1403003"
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<ul>
 
<ul>
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Constitutive promoter
+
<li>Constitutive promoter</li>
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Synthetic RBS  
+
<li>Synthetic RBS</li>
−
Limonene synthetase (LIMS1)  
+
<li>Limonene synthetase (LIMS1)</li>
 
</ul>
 
</ul>
  
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
 
Transform <i>E. coli</i> for (+)-Limonene biosynthesis.
 
Transform <i>E. coli</i> for (+)-Limonene biosynthesis.
  

Revision as of 10:56, 18 October 2014

Limonene synthase (LIMS1) expression cassette

  • Constitutive promoter
  • Synthetic RBS
  • Limonene synthetase (LIMS1)

Transform E. coli for (+)-Limonene biosynthesis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 82
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 187


Verification

Limonene.png

Expected bands at 2kbp for pSB1C3 and 1798bp for the limonene generator