Difference between revisions of "Part:BBa J58100"

Latest revision as of 21:17, 29 October 2006

AND-type promoter synergistically activated by cI and CRP

We have designed a promoter synergistically activated by two transcription factors. We have used the part BBa_I12007 as starting point, together with the ideas of the paper by Joung et al. Two heterologous prokariotic activators, the bacteriophage lambda cI protein, and the E. coli cyclic AMP receptor protein (CRP), activate transcription synergistically from an artificial promoter bearing binding site for both proteins. Both proteins interact directly with RNA polymerase and bind DNA at two operator sites.

By Valencia iGEM 2006

A. Aparici (2), MC. Aroca (2), J. Carrera (1), C. Edo (1,2), G. Fuertes (2), D. Jimenez (2), C. Mata (2), JV. Medrano (2), A. Montagud (2), C. Navarrete (2), E. Navarro (1), G. Rodrigo (1), M. Baguena (1), P. Tortosa (3), P. Fernandez-de-Cordoba (1), A. Ferrando (2), J. Salgado (2), J. Urchueguia (1), A. Jaramillo (3).

1 Universidad Politecnica de Valencia, Spain

2 Universitat de Valencia, Spain

3 Ecole Polytechnique, France

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 11
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 11
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 11
1000
COMPATIBLE WITH RFC[1000]

The CRP binding site contains an XbaI site that could not be removed without compromising the promoter's activity. Avoid using XbaI when using this part. A new part is under development that will have a mutated but functional CRP binding site.

@@ Line 2: / Line 2: @@
 <partinfo>BBa_J58100 short</partinfo>
-Two heterologous prokariotic activators, the bacteriophage lambda cI protein, and the E. coli cyclic AMP receptor protein (CRP), were shown to activate transcription synergistically from an artificial promoter bearing binding site for both proteins. The synergy depends on a functional activation (positive control) surface on each activator. Thus, both proteins interact directly with RNA polymerase.
+We have designed a promoter synergistically activated by two transcription factors. We have used the part <partinfo>I12007</partinfo> as starting point, together with the ideas of the paper by Joung et al. Two heterologous prokariotic activators, the bacteriophage lambda cI protein, and the E. coli cyclic AMP receptor protein (CRP),  activate transcription synergistically from an artificial promoter bearing binding site for both proteins. Both proteins interact directly with RNA polymerase and bind DNA at two operator sites.
-A. Aparici (2), MC. Aroca (2), J. Carrera (1), C. Edo (1,2), G. Fuertes (2), D. Jimenez (2), C. Mata (2), JV. Medrano (2), A. Montagud (2), C. Navarrete (2), E. Navarro (1), G. Rodrigo (1), M. Baguena (1), P. Tortosa (3), P. Fernandez-de-Cordoba (1), A. Ferrando (2), J. Salgado (2), J. Urchueguia (1), A. Jaramillo (3).
+'''By Valencia iGEM 2006'''
-Universidad Politecnica de Valencia, Spain
-Universitat de Valencia, Spain
+''A. Aparici (2), MC. Aroca (2), J. Carrera (1), C. Edo (1,2), G. Fuertes (2), D. Jimenez (2), C. Mata (2), JV. Medrano (2), A. Montagud (2), C. Navarrete (2), E. Navarro (1), G. Rodrigo (1), M. Baguena (1), P. Tortosa (3), P. Fernandez-de-Cordoba (1), A. Ferrando (2), J. Salgado (2), J. Urchueguia (1), A. Jaramillo (3).''
-Ecole Polytechnique, France
+''1 Universidad Politecnica de Valencia, Spain''
+''2 Universitat de Valencia, Spain''
+''3 Ecole Polytechnique, France''
@@ Line 16: / Line 21: @@
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_J58100 SequenceAndFeatures</partinfo>
+The CRP binding site contains an XbaI site that could not be removed without compromising the promoter's activity. Avoid using XbaI when using this part. A new part is under development that will have a mutated but functional CRP binding site.
 <!-- Uncomment this to enable Functional Parameter display