Difference between revisions of "Part:BBa K1431412"
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This three plasmid(with BBa_K1431411 and BBa_K1431413) carry the Target-sequence aim to check if the CRISPR-Cas9 system we create in human cell can work well, which might be the foundation for the further experiments. . | This three plasmid(with BBa_K1431411 and BBa_K1431413) carry the Target-sequence aim to check if the CRISPR-Cas9 system we create in human cell can work well, which might be the foundation for the further experiments. . | ||
Latest revision as of 03:56, 18 October 2014
target sequence1 for HBV
Introduction
This three plasmid(with BBa_K1431411 and BBa_K1431413) carry the Target-sequence aim to check if the CRISPR-Cas9 system we create in human cell can work well, which might be the foundation for the further experiments. .
However, although each one with different target-sequence, they still have plenty of components in common. You can see, all of them carry at least one section of target-sequences, and target-sequence for HBV1-HBV2 have two sections of target-sequence.This is because this part plays an important role in efficiency test, both for target-sequence binding gRNA and cut-off ability of CRISPR-Cas9.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]