Difference between revisions of "Part:BBa K1491021"
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<p>Of the three superoxide generators, Supernova had the biggest decrease in viability following photobleaching when compared to its control. It also had the largest decrease in fluorescence following photobleaching. Fluorescence was measured at (ex/em = 585nm/610nm).</p> | <p>Of the three superoxide generators, Supernova had the biggest decrease in viability following photobleaching when compared to its control. It also had the largest decrease in fluorescence following photobleaching. Fluorescence was measured at (ex/em = 585nm/610nm).</p> | ||
| + | <center>https://static.igem.org/mediawiki/2014/thumb/d/dc/ConstitutiveSuperoxides.jpg/386px-ConstitutiveSuperoxides.jpg</center> | ||
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Latest revision as of 03:54, 18 October 2014
Constitutive Codon Optimized Super Nova
Codon optimized version of the monomeric superoxide generator Super Nova (BBa_K1491017) with a constitutive promoter (BBa_J23100) and a ribosome binding site (BBa_B0034). Super Nova is the monomeric form of Killer Red (BBa_K1184000).
Of the three superoxide generators, Supernova had the biggest decrease in viability following photobleaching when compared to its control. It also had the largest decrease in fluorescence following photobleaching. Fluorescence was measured at (ex/em = 585nm/610nm).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 111
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]