Difference between revisions of "Part:BBa K1344001"

 
 
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<partinfo>BBa_K1344001 short</partinfo>
 
<partinfo>BBa_K1344001 short</partinfo>
  
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This part will expressing 1018 by inducing IPTG at various concentration
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This part will expressing [https://parts.igem.org/Part:BBa_K1344000 Peptide 1018] by lac promoter (inducing with IPTG)
  
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===Usage and Biology===
 
===Usage and Biology===
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Peptide 1018 is small cationic peptide under T5 promoter (inducible promoter). Peptide 1018 in particular concentration can be toxic for bacterial vector. Isopropyl thiogalactoside (IPTG) is analogue of lactose that is able to induce activation of T5 promoter to start transcription.
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<br><br>
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<b>Aim</b><br>
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To examine in which concentration of IPTG that can be toxic for <i>E.coli</i> as bacterial vector
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<br><br>
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<b>Result</b><br><br>
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"https://static.igem.org/mediawiki/2014/9/90/Antimicrobial_result.png"
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"https://static.igem.org/mediawiki/2014/6/64/Antimicrobial_assay.png"
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<br><br>
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<b>Discussion</b><br>
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From the experiment above we expected that IPTG induction will activate peptide 1018 trancription which is able to inhibit the growth of <i>E.coli</i> and can reduce the number of cells if the cells grow together with IPTG in the start point. In this experiment we the activation was conducted when OD 0.1 and dilution 1:20 without incubation before. The data above show no inhibitory effect of IPTG as increase the concentration. But when we measured the OD600, all treatment show lower in OD600 of IPTG 0 mM. We assumed that the peptide 1018 still working, even though we need more data and incubation period before treated with IPTG.
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Latest revision as of 03:36, 18 October 2014

T5-lac-1018

This part will expressing Peptide 1018 by lac promoter (inducing with IPTG)

Usage and Biology

Peptide 1018 is small cationic peptide under T5 promoter (inducible promoter). Peptide 1018 in particular concentration can be toxic for bacterial vector. Isopropyl thiogalactoside (IPTG) is analogue of lactose that is able to induce activation of T5 promoter to start transcription.

Aim
To examine in which concentration of IPTG that can be toxic for E.coli as bacterial vector

Result

"Antimicrobial_result.png" "Antimicrobial_assay.png"

Discussion
From the experiment above we expected that IPTG induction will activate peptide 1018 trancription which is able to inhibit the growth of E.coli and can reduce the number of cells if the cells grow together with IPTG in the start point. In this experiment we the activation was conducted when OD 0.1 and dilution 1:20 without incubation before. The data above show no inhibitory effect of IPTG as increase the concentration. But when we measured the OD600, all treatment show lower in OD600 of IPTG 0 mM. We assumed that the peptide 1018 still working, even though we need more data and incubation period before treated with IPTG.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]