Difference between revisions of "Part:BBa K1509003"

 
 
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<partinfo>BBa_K1509003 short</partinfo>
 
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The synthetic CP25 promoter was designed to be constitutive. CP25 is a stronge promoter in Lactococcus lactis by Jensen and Hammer.In the article,they measure the activities of the CP promoter in E. coli.The promoter activities were assayed from the expression of a reporter gene (lacLM) encoding &#946;-galactosidase transcribed from the different synthetic promoter clones on the promoter cloning vector pAK80.The result suggests CP25 is the strongest promoter.
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The synthetic CP25 promoter was designed to be constitutive. CP25 is a stronge promoter in Lactococcus lactis by Jensen and Hammer.In the article,they measured the activities of the CP promoter in <em>E. coli</em>.The promoter activities were assayed from the expression of a reporter gene (lacLM) encoding &#946;-galactosidase transcribed from the different synthetic promoter clones on the promoter cloning vector pAK80.The result suggests CP25 is the strongest promoter.
  
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===Usage and Biology===
 
===Usage and Biology===
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[[File:Nanocrystal.png]]
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fig1.CDS7 gene was under control of CP25 promoter.
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===Result===
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[[File:Nanocrystal3.png]]
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fig2.Our recombinant ''E.coli'' in the feild of TEM.
  
 
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Latest revision as of 03:35, 18 October 2014

CP25 is a highly active,constitutive promoter

The synthetic CP25 promoter was designed to be constitutive. CP25 is a stronge promoter in Lactococcus lactis by Jensen and Hammer.In the article,they measured the activities of the CP promoter in E. coli.The promoter activities were assayed from the expression of a reporter gene (lacLM) encoding β-galactosidase transcribed from the different synthetic promoter clones on the promoter cloning vector pAK80.The result suggests CP25 is the strongest promoter.

Usage and Biology

Nanocrystal.png


fig1.CDS7 gene was under control of CP25 promoter.

Result

Nanocrystal3.png


fig2.Our recombinant E.coli in the feild of TEM.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]