Difference between revisions of "Part:BBa K1366104"

Latest revision as of 03:34, 18 October 2014

Apoptin expression under a CMV promoter (C5)

This construct will be used to characterize the transfection of a death effector (apoptin) in mammalian cells mediated by bacteriophages. It contains the hTERT promoter, specific for cancerous cells lines. The construct will be contained in a phagemid with de f1 origin of replication (for single stranded DNA), and it is intended to replace the M13 genome. Apoptin will be codon optimized for mammalian cell line.

In the next image it is presented the restriction digestion analysis of some biobricks:

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1428

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1366104 short</partinfo>
 This construct will be used to characterize the transfection of a death effector (apoptin) in mammalian cells mediated by bacteriophages. It contains the hTERT promoter, specific for cancerous cells lines. The construct will be contained in a phagemid with de f1 origin of replication (for single stranded DNA), and it is intended to replace the M13 genome. Apoptin will be codon optimized for mammalian cell line.
+In the next image it is presented the restriction digestion analysis of some biobricks:
+<center>
+https://static.igem.org/mediawiki/2014/2/2b/Gel_psb1c3.png
+</center>
 <!-- Add more about the biology of this part here