Difference between revisions of "Part:BBa K1366103"

 
 
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<partinfo>BBa_K1366103 short</partinfo>
 
<partinfo>BBa_K1366103 short</partinfo>
  
 
This composite act as a probe of concept of the transfection in mammalian cells mediated by bacteriophages. It contains the hTERT promoter, specific for cancerous cells lines. The construct will be contained in a phagemid with de f1 origin of replication (for single stranded DNA), and it is intended to replace the M13 genome. The GFP will be codon optimized for mammalian cell line.
 
This composite act as a probe of concept of the transfection in mammalian cells mediated by bacteriophages. It contains the hTERT promoter, specific for cancerous cells lines. The construct will be contained in a phagemid with de f1 origin of replication (for single stranded DNA), and it is intended to replace the M13 genome. The GFP will be codon optimized for mammalian cell line.
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In the next image it is presented the restriction digestion analysis of some biobricks:
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<center>
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https://static.igem.org/mediawiki/2014/2/2b/Gel_psb1c3.png
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</center>
  
  

Latest revision as of 03:33, 18 October 2014

GFP expression under a hTERT promoter (C4)

This composite act as a probe of concept of the transfection in mammalian cells mediated by bacteriophages. It contains the hTERT promoter, specific for cancerous cells lines. The construct will be contained in a phagemid with de f1 origin of replication (for single stranded DNA), and it is intended to replace the M13 genome. The GFP will be codon optimized for mammalian cell line.

In the next image it is presented the restriction digestion analysis of some biobricks:

Gel_psb1c3.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1572