Difference between revisions of "Part:BBa K1498000:Experience"
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Under the control of the constitutive promoter BBa_J23110 and following expression in ''E. coli'' BL21 the supernatant was analysed on a SDS-PAGE gel. Following coomassie staining a protein of around 36 kDa was visibly overexpressed -this size is smaller than the unprocessed keratinase, but larger than the fully processed protein suggesting that a pre-processed version of the protein was present. However only the processed form of the protein will exhibit activity. | Under the control of the constitutive promoter BBa_J23110 and following expression in ''E. coli'' BL21 the supernatant was analysed on a SDS-PAGE gel. Following coomassie staining a protein of around 36 kDa was visibly overexpressed -this size is smaller than the unprocessed keratinase, but larger than the fully processed protein suggesting that a pre-processed version of the protein was present. However only the processed form of the protein will exhibit activity. | ||
| + | https://static.igem.org/mediawiki/2014/d/d5/Kerat2_Sheffield2014.png | ||
| + | <br> | ||
| + | Lane 1: EZ run protein ladder (Orange marker 72kDa, then 55, 40 and 33 kDa). | ||
| + | <br> | ||
| + | Lane 3: Secreted keratinase | ||
| + | <br> | ||
| + | |||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 03:21, 18 October 2014
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Applications of BBa_K1498000
Under the control of the constitutive promoter BBa_J23110 and following expression in E. coli BL21 the supernatant was analysed on a SDS-PAGE gel. Following coomassie staining a protein of around 36 kDa was visibly overexpressed -this size is smaller than the unprocessed keratinase, but larger than the fully processed protein suggesting that a pre-processed version of the protein was present. However only the processed form of the protein will exhibit activity.
Lane 1: EZ run protein ladder (Orange marker 72kDa, then 55, 40 and 33 kDa).
Lane 3: Secreted keratinase
User Reviews
UNIQ488669dd261fc9f6-partinfo-00000000-QINU UNIQ488669dd261fc9f6-partinfo-00000001-QINU