Difference between revisions of "Part:BBa K1498000:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K1498000=== | ===Applications of BBa_K1498000=== | ||
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| + | Under the control of the constitutive promoter BBa_J23110 and following expression in ''E. coli'' BL21 the supernatant was analysed on a SDS-PAGE gel. Following coomassie staining a protein of around 36 kDa was visibly overexpressed -this size is smaller than the unprocessed keratinase, but larger than the fully processed protein suggesting that a pre-processed version of the protein was present. However only the processed form of the protein will exhibit activity. | ||
| + | https://static.igem.org/mediawiki/2014/d/d5/Kerat2_Sheffield2014.png | ||
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| + | Lane 1: EZ run protein ladder (Orange marker 72kDa, then 55, 40 and 33 kDa). | ||
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| + | Lane 3: Secreted keratinase | ||
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===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 03:21, 18 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1498000
Under the control of the constitutive promoter BBa_J23110 and following expression in E. coli BL21 the supernatant was analysed on a SDS-PAGE gel. Following coomassie staining a protein of around 36 kDa was visibly overexpressed -this size is smaller than the unprocessed keratinase, but larger than the fully processed protein suggesting that a pre-processed version of the protein was present. However only the processed form of the protein will exhibit activity.
Lane 1: EZ run protein ladder (Orange marker 72kDa, then 55, 40 and 33 kDa).
Lane 3: Secreted keratinase
User Reviews
UNIQd31c488064ada1af-partinfo-00000000-QINU UNIQd31c488064ada1af-partinfo-00000001-QINU