Difference between revisions of "Part:BBa K1465103"

 
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===Usage and Biology===
 
===Usage and Biology===
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Fumarate reductase is part of the anaerobic fumarate respiration in bacteria. It catalyzes the reaction from fumarate into succinate using fumarate as a final electron acceptor in anaerobic fumarate respiration. The related enzyme in aerobic respiration is the succinate dehydrogenase, which catalyses the reaction from succinate to fumarate.
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The electrons are transferred from succinate to FAD<sup>+</sup> producing fumarate and FADH<sub>2</sub>. The succinate dehydrogenase is also a membrane enzyme and it is part of the citric acid cycle. They both belong to the respiration complex II. Naturally there is no activity of both enzymes at the same time in <i>E. coli</i> cells. (<a href="#Iverson1999">Iverson <i>et al.</i>, 1999</a>)<br>
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In our project we used the fumarate reductase in combination with an extracellular mediator as electron donor to transfer electrons into bacterial cells. The reduced mediator crosses the outer membrane of <i>E. coli</i> through the outer membrane porine OprF (<a href="https://parts.igem.org/Part:BBa_K1172507">BBa_K1172507</a>).
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Mediators adsorb at the inner membrane and transfer electrons to the fumarate reductase. After that the reduced fumarate reductase transfer electrons to fumarate producing succinate. This process has been shown for the fumarate reductase in <i>Actinobacillus succinogenes</i> by <a href="#Park1999">Park <i>et al.</i></a>. <br>
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As published by <a href="#Park1999">Park <i>et al.</i>, 1999</a> the fumarate reductase from <i>A. succinogenes</i> shows high activity accepting electrical reduced neutral red as an electron donor and so stimulates the metabolism in a electrobiochemical reactor system. We planed to compare activity of fumarate reductase Fum of <i>A. succinogenes</i> with fumarate reductase Frd of <i>E. coli</i> (a href="https://parts.igem.org/Part:BBa_K1465102">BBa_K1465102) with regard to activity accepting neutral red or bromphenol blue as alternative electron acceptors in our <a href="http://2014.igem.org/Team:Bielefeld-CeBiTec/Results/rMFC/Construction#H-cellresults">electrobiochemical reactor system</a>.
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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===References===
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    Iverson <i>et al.</i>, 1999. Structure of the <i>Escherichia coli</i> Fumarate Reductase Respiratory Complex. <a href="http://www.sciencemag.org/content/284/5422/1961.short" target="_blank">Science, vol. 284, pp. 1961-1966</a>
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    Park <i>et al.</i>, 1999. Utilization of Electrically Reduced Neutral Red byActinobacillus succinogenes: Physiological Function of Neutral Red in Membrane-Driven Fumarate Reduction and Energy Conservation. <a href="http://onlinelibrary.wiley.com/doi/10.1002/food.19870310532/abstract" target="_blank">Journal of Bacteriology, vol. 181, pp. 2403-2410</a>
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Revision as of 03:14, 18 October 2014

Fumarate reductase subunit A (FumA) from Actinobacillus succinogenes

Fumerate reductase (FumABCD) from Actinobacillus succinogenes

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 734
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1120


References