Difference between revisions of "Part:BBa K1366105"
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<partinfo>BBa_K1366105 short</partinfo> | <partinfo>BBa_K1366105 short</partinfo> | ||
This construct will be used to characterize the transfection of an effector of death (Survivin siRNA) in mammalian cells mediated by bacteriophages. It contains the hTERT promoter, specific for cancerous cells lines. The construct will be contained in a phagemid with de f1 origin of replication (for single stranded DNA), and it is intended to replace the M13 genome. | This construct will be used to characterize the transfection of an effector of death (Survivin siRNA) in mammalian cells mediated by bacteriophages. It contains the hTERT promoter, specific for cancerous cells lines. The construct will be contained in a phagemid with de f1 origin of replication (for single stranded DNA), and it is intended to replace the M13 genome. | ||
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| + | In the next image it is presented the restriction digestion analysis of some biobricks: | ||
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| + | https://static.igem.org/mediawiki/2014/2/2b/Gel_psb1c3.png | ||
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Revision as of 02:48, 18 October 2014
Production of survivin siRNA under the hTERT promoter (C6)
This construct will be used to characterize the transfection of an effector of death (Survivin siRNA) in mammalian cells mediated by bacteriophages. It contains the hTERT promoter, specific for cancerous cells lines. The construct will be contained in a phagemid with de f1 origin of replication (for single stranded DNA), and it is intended to replace the M13 genome.
In the next image it is presented the restriction digestion analysis of some biobricks:
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 901