Difference between revisions of "Part:BBa K1365020"

 
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<partinfo>BBa_K1365020 short</partinfo>
 
<partinfo>BBa_K1365020 short</partinfo>
  
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Codes for the sfGFP protein, an optimized fluorescent reporter. Was originally optimized for Bacillus subtilis, but then showed high fluorescence in Lactococcus lactis.
 
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===Usage and Biology===
 
===Usage and Biology===
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This gene codes for the sfGFP(Bs) protein, an optimized fluorescent reporter. This sfGFP was originally optimized for ''Bacillus subtilis''. When it was tested in different species it showed high fluorescence in ''Lactococcus lactis''. The sfGFP(Bs) has a very high performance in ''L. lactis'' compared to other GFP proteins, and also works really well in other bacteria like ''B. subtilis'', ''Escherischia coli'' and ''Streptococcus pneumoniae''.
  
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[[File:sfgfpbs.png]]
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and features===
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<span class='h3bb'></span>
 
<partinfo>BBa_K1365020 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1365020 SequenceAndFeatures</partinfo>
  
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===References===
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Overkamp, W. et al. (2013) Benchmarking various green fluorescent protein variants in ''Bacillus subtilis'', ''Streptococcus pneumoniae'', and ''Lactococcus lactis'' for live cell imaging. ''Appl. Environ. Microbiol.'' 79: 6481-6490
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
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Revision as of 02:48, 18 October 2014

sfGFP(Bs)

Usage and Biology

This gene codes for the sfGFP(Bs) protein, an optimized fluorescent reporter. This sfGFP was originally optimized for Bacillus subtilis. When it was tested in different species it showed high fluorescence in Lactococcus lactis. The sfGFP(Bs) has a very high performance in L. lactis compared to other GFP proteins, and also works really well in other bacteria like B. subtilis, Escherischia coli and Streptococcus pneumoniae.

Sfgfpbs.png

Sequence and features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13


References

Overkamp, W. et al. (2013) Benchmarking various green fluorescent protein variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for live cell imaging. Appl. Environ. Microbiol. 79: 6481-6490