Difference between revisions of "Part:BBa K1439004:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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+ | [[File:OUC-China_K1439004_Experience1.png]] | ||
+ | ====Fig 1.==== | ||
+ | Fig 1.We wanted to know if the fusion protein will combine the plasimd. We mixed up the protein TAT:H4 with the plasmid pcDNA3.1(+),and the mass ratios of DNA and protein were 1:0,1:1, 1:2, 1:4, 1:6, 1:8 in 2ul glucose solution. This picture showed that TAT-H4 can combine with DNA, because the plasmid was retraded. | ||
+ | [[File:OUC-China_K1439004_Experience2.png]] | ||
+ | ====Fig 2.==== | ||
+ | Fig2.We wanted to test if the fusion protein can protect the plasmid, we used DNaseI deal with the complex that the protein TAT:H4 with the plasmid pcDNA3.1(+),and the mass ratios of DNA and protein were 1:0,1:1, 1:2, 1:4, 1:6, 1:8 in 2ul glucose solution. This picture shows that the fusion protein can protect the plasmid. |
Latest revision as of 02:48, 18 October 2014
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
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Fig 1.
Fig 1.We wanted to know if the fusion protein will combine the plasimd. We mixed up the protein TAT:H4 with the plasmid pcDNA3.1(+),and the mass ratios of DNA and protein were 1:0,1:1, 1:2, 1:4, 1:6, 1:8 in 2ul glucose solution. This picture showed that TAT-H4 can combine with DNA, because the plasmid was retraded.
Fig 2.
Fig2.We wanted to test if the fusion protein can protect the plasmid, we used DNaseI deal with the complex that the protein TAT:H4 with the plasmid pcDNA3.1(+),and the mass ratios of DNA and protein were 1:0,1:1, 1:2, 1:4, 1:6, 1:8 in 2ul glucose solution. This picture shows that the fusion protein can protect the plasmid.