Difference between revisions of "Part:BBa K1400002"

 
 
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<partinfo>BBa_K1400002 short</partinfo>
 
<partinfo>BBa_K1400002 short</partinfo>
  
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A lower expression pGAL promoter with 4 gal4 sites. This is used to have similar expression as pGALTX and PTREGX. It is a single input promoter, with scrambled mig1 sites.  
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This is the native pGAL1 promoter in S. cerevisiae with its Mig1 sites removed. This is a single input promoter that has four upstream activating sequences (UAS), which are GAL4 binding elements. The Mig1 sites are binding sites for proteins responsible for transcriptional repression of downstream genes to this and other promoters in S. cerevisiae in the presence of glucose. The removal of the Mig1 sites allows transcriptional activation of the promoter in the presence of glucose in the cellular growth medium.
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<html><img style="width:55%;" src="https://static.igem.org/mediawiki/2014/1/1c/Pgal4sites.png" /></html>
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Figure 1: Characterization of pGAL via dual drug induction. pGAL has no repressing sites and 4 activating GAL4 sites, so increasing estradiol increases activation while aTc has no effect beyond auto-fluorescence. The fusion protein, GEV (GAL4 binding domain-human estrogen receptor-VP16 activator domain), is the activator and is expressed with the weak constitutive promoter, pMRP7.
  
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><b>Sequence and Features</b></span>
 
<partinfo>BBa_K1400002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1400002 SequenceAndFeatures</partinfo>
  

Latest revision as of 02:45, 18 October 2014

PGAL Modified native pGAL

This is the native pGAL1 promoter in S. cerevisiae with its Mig1 sites removed. This is a single input promoter that has four upstream activating sequences (UAS), which are GAL4 binding elements. The Mig1 sites are binding sites for proteins responsible for transcriptional repression of downstream genes to this and other promoters in S. cerevisiae in the presence of glucose. The removal of the Mig1 sites allows transcriptional activation of the promoter in the presence of glucose in the cellular growth medium.

Figure 1: Characterization of pGAL via dual drug induction. pGAL has no repressing sites and 4 activating GAL4 sites, so increasing estradiol increases activation while aTc has no effect beyond auto-fluorescence. The fusion protein, GEV (GAL4 binding domain-human estrogen receptor-VP16 activator domain), is the activator and is expressed with the weak constitutive promoter, pMRP7.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 76
  • 1000
    COMPATIBLE WITH RFC[1000]