Difference between revisions of "Part:BBa K1520521"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1520521 short</partinfo> | <partinfo>BBa_K1520521 short</partinfo> | ||
PgolTS-golS-PgolB-tetR-Ter-PtetO-rfp-Ter-Plac-tetR-Ter-Pcons2-lacI-Ter | PgolTS-golS-PgolB-tetR-Ter-PtetO-rfp-Ter-Plac-tetR-Ter-Pcons2-lacI-Ter | ||
| − | It is | + | |
| + | PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter | ||
| + | It is a test of our suicide circuit for gold ions. | ||
| + | It can express rfp when both gold ions and IPTG are not exist. Here rfp represent lysozyme, since the plasmid with lysozyme is hard to reconstruct. When the bacteria turns red, it means they commit suicide and the following pictures shows that the system work. | ||
| + | |||
| + | [[File:goldandiptg.jpg]] | ||
| + | |||
| + | From left to right: 1.add gold 2.without gold 3.add IPTG 4.without IPTG | ||
| + | |||
| + | [[File:Fluo.jpg]] | ||
| + | |||
| + | The fluorescence intensity of each treatment | ||
| + | |||
| + | [[File:k1520509.jpg]] | ||
| + | |||
| + | From left to right, Marker(1Kb), | ||
| + | |||
| + | PgolTS-golB-PgolB-tetR-Ter-Ptet-rbs-rfp-Ter, | ||
| + | |||
| + | PgolTS-golB-PgolB-tetR-Ter-Ptet-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter. | ||
| + | |||
| + | The substances of electrophoresis are made from PCR of plasmid with VF2 and VR as their primers. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 02:14, 18 October 2014
PgolTS-golS-PgolB-tetR-Ter-PtetO-rfp-Ter-Plac-tetR-Ter-Pcons2-lacI-Ter
PgolTS-golS-PgolB-tetR-Ter-PtetO-rfp-Ter-Plac-tetR-Ter-Pcons2-lacI-Ter
PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter It is a test of our suicide circuit for gold ions. It can express rfp when both gold ions and IPTG are not exist. Here rfp represent lysozyme, since the plasmid with lysozyme is hard to reconstruct. When the bacteria turns red, it means they commit suicide and the following pictures shows that the system work.
From left to right: 1.add gold 2.without gold 3.add IPTG 4.without IPTG
The fluorescence intensity of each treatment
From left to right, Marker(1Kb),
PgolTS-golB-PgolB-tetR-Ter-Ptet-rbs-rfp-Ter,
PgolTS-golB-PgolB-tetR-Ter-Ptet-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter.
The substances of electrophoresis are made from PCR of plasmid with VF2 and VR as their primers.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 3611
Illegal NheI site found at 3634 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 4812
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 548
- 1000COMPATIBLE WITH RFC[1000]