Difference between revisions of "Part:BBa K1365000"

Revision as of 02:10, 18 October 2014

NisA

Usage and Biology

NisA codes for the nisin precursor. Together with the genes NisB, NisT, NisC, NisP, NisR and NisK of the nisin operon it is responsible for producing the lantibiotic nisin in Lactococcus lactis, see the figure below. The NisA protein is first modified and then transported out of the cell. The serines and threonines of NisA are dehydrated by NisB and then the precursor is cyclized by NisC. After this process, the precursor is transported out of the cell (2). Here, the lead peptide is cut off by NisP (3) and the mature nisin is formed.¹

Nisin is a lantibiotic, an bacteriocidal peptide. Nisin inhibits the growth of a broad range of Gram positive bacteria, of which many are spoilage bacteria or pathogens. Nisin is therefore extensively used in the food industry as a preservative. Nisin forms pores in the membrane of the bacteria it kills and inhibits the peptidoglycan synthesis.²

The nisA gene has a nice functionality in the strain L. lactis NZ9800. This strain contains the transposon Tn5276 which carries the complete nisin A operon. The strain has a deletion in its original nisA gene, that makes the gene non functional. This BioBrick can therefore be used in L. lactis NZ9800 to let it produce nisin again.

Sequence and features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

1. Cheigh, C. I. and Pyun, Y.R. (2005) Nisin biosynthesis and its properties. Biotechnol. Lett. 27: 1641-1648
2. Zhou, H. et al. (2014) Mechanisms of nisin resistance in Gram-positive bacteria. Ann. Microbiol. 64: 413-420

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 ===Usage and Biology===
-NisA codes for the nisin precursor. Together with the genes NisB, NisT, NisC, NisP, NisR and NisK of the nisin operon it is responsible for producing the lantibiotic nisin in ''Lactococcus lactis'', see the figure below. The NisA protein is first modified and then transported out of the cell. The serines and threonines of NisA are dehydrated by NisB and then the precursor is cyclized by NisC. After this process, the precursor is transported out of the cell (2). Here, the lead peptide is cut off by NisP (3) and the mature nisin is formed. [1]
+NisA codes for the nisin precursor. Together with the genes NisB, NisT, NisC, NisP, NisR and NisK of the nisin operon it is responsible for producing the lantibiotic nisin in ''Lactococcus lactis'', see the figure below. The NisA protein is first modified and then transported out of the cell. The serines and threonines of NisA are dehydrated by NisB and then the precursor is cyclized by NisC. After this process, the precursor is transported out of the cell (2). Here, the lead peptide is cut off by NisP (3) and the mature nisin is formed.<sup>1</sup>
 [[File:Nisin_casette.art.png]]
-Nisin is a lantibiotic, an bacteriocidal peptide. Nisin inhibits the growth of a broad range of Gram positive bacteria, of which many are spoilage bacteria or pathogens. Nisin is therefore extensively used in the food industry as a preservative. Nisin forms pores in the membrane of the bacteria it kills and inhibits the peptidoglycan synthesis. [2]
+Nisin is a lantibiotic, an bacteriocidal peptide. Nisin inhibits the growth of a broad range of Gram positive bacteria, of which many are spoilage bacteria or pathogens. Nisin is therefore extensively used in the food industry as a preservative. Nisin forms pores in the membrane of the bacteria it kills and inhibits the peptidoglycan synthesis.<sup>2</sup>
 The nisA gene has a nice functionality in the strain ''L. lactis'' NZ9800. This strain contains the transposon Tn5276 which carries the complete nisin A operon. The strain has a deletion in its original nisA gene, that makes the gene non functional. This BioBrick can therefore be used in ''L. lactis'' NZ9800 to let it produce nisin again.