Difference between revisions of "Part:BBa K1372001"

Revision as of 02:08, 18 October 2014

Salicylate Inducible Suppressing System

This Biobrick is the result of the assembly of two BB:

- BBa_K1372000:

- BBa_B0015: Double terminator consisting of BBa_B0010 and BBa_B0012.

Thus, there will be a terminator of the transcription of tRNA-SUPD gene (missing in BBa_K1372000).

This Bioibrick could thus be used as control system of the production of tRNA-SUPD depending on the concentration of a substrate (salicylate) gradually consumed by the organisms in the growth-medium. Consequentially, the production of tRNA-SupD will gradually peter out.

An example of use of this control system is described there: [http://2014.igem.org/Team:Paris_Saclay/Project/Salicylate_Inducible_System Salicylate Inducible System]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 786
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 77
Illegal NgoMIV site found at 618
Illegal AgeI site found at 1309
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1312

Made by [http://2014.igem.org/Team:Paris_Saclay iGEM Paris Saclay]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1372001 short</partinfo>
-This BB is the result of the assembly of two BB:
+This Biobrick is the result of the assembly of two BB:
 - [https://parts.igem.org/Part:BBa_K1372000 BBa_K1372000]:
@@ Line 14: / Line 14: @@
 [[File:Paris Saclay BB2001.png|center]]
-This BB could thus be used as control system of the production of tRNA-SUPD depending on the concentration of a substrate (salicylate) gradually consumed by the organisms in the growth-medium. Consequentially, the production of tRNA-SupD will gradually peter out.
+This Bioibrick could thus be used as control system of the production of tRNA-SUPD depending on the concentration of a substrate (salicylate) gradually consumed by the organisms in the growth-medium. Consequentially, the production of tRNA-SupD will gradually peter out.
 An example of use of this control system is described there: [http://2014.igem.org/Team:Paris_Saclay/Project/Salicylate_Inducible_System Salicylate Inducible System]