Difference between revisions of "Part:BBa K1431201"

Latest revision as of 01:54, 18 October 2014

NLS, lead protein into the nucleus

A protein tags with a nuclear localization signal or sequence(NLS) will bind strongly to importin (aka karyopherin), and, together, the complex will move through the nuclear pore.That is very useful to localize protein or make a sign in cell nucleus.

We design two NLS which connect by dozens of nucleotides and can be inserted a coding sequence by BbsI site. See the mechanism of BbsI site work in the design page. The two NLS in each side of a protein may increase the probability of protein import into the cell nucleus.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 66
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 88
Illegal NgoMIV site found at 107
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1431201 short</partinfo>
-A nuclear localization signal or sequence (NLS) is an amino acid sequence that "tags" a protein for import into the cell nucleus by nuclear transport. A protein translated with a NLS will bind strongly to importin (aka karyopherin), and, together, the complex will move through the nuclear pore.
+A protein tags with a nuclear localization signal or sequence(NLS) will bind strongly to importin (aka karyopherin), and, together, the complex will move through the nuclear pore.That is very useful to localize protein or make a sign in cell nucleus.
-We design two NLS which connect by dozens of nucleotides and can be inserted a coding sequence by BbsI site. The two NLS in each side of a protein may increase the probability of protein import into the cell nucleus.
+We design two NLS which connect by dozens of nucleotides and can be inserted a coding sequence by BbsI site. See the mechanism of BbsI site work in the [https://parts.igem.org/Part:BBa_K1431201:Design design page]. The two NLS in each side of a protein may increase the probability of protein import into the cell nucleus.
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