Difference between revisions of "Part:BBa J58101"
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_J58101 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_J58101 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 08:09, 29 October 2006

Promoter activated synergistically by cI and CRP with the reporter RFP

This composed part contains a two-regulator AND promoter activated synergistically by cI and CRP proteins:
pAND
J58100
joined to a generator of the reporter RFP

I13507
The deep components are as follows:
pAND
J58100
B0034		mRFP1
E1010
B0010
B0012
With this construction, we obtain a genetic device (not using POPS) that produces RFP when enough levels of cI and CRP are acheived simultaneously.

By Valencia iGEM 2006

A. Aparici (2), MC. Aroca (2), J. Carrera (1), C. Edo (1,2), G. Fuertes (2), D. Jimenez (2), C. Mata (2), JV. Medrano (2), A. Montagud (2), C. Navarrete (2), E. Navarro (1), G. Rodrigo (1), M. Baguena (1), P. Tortosa (3), P. Fernandez-de-Cordoba (1), A. Ferrando (2), J. Salgado (2), J. Urchueguia (1), A. Jaramillo (3).

1 Universidad Politecnica de Valencia, Spain

2 Universitat de Valencia, Spain

3 Ecole Polytechnique, France


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 11
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 11
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 11
    Illegal AgeI site found at 687
    Illegal AgeI site found at 799
  • 1000
    COMPATIBLE WITH RFC[1000]

See comments in AND-type promoter synergistically activated by cI and CRP