Difference between revisions of "Part:BBa K1491021"

Revision as of 01:10, 18 October 2014

Constitutive Codon Optimized Super Nova

Codon optimized version of the monomeric superoxide generator Super Nova (BBa_K1491017) with a constitutive promoter (BBa_J23100) and a ribosome binding site (BBa_B0034). Super Nova is the monomeric form of Killer Red (BBa_K1184000).

Of the three superoxide generators, Supernova had the biggest decrease in viability following photobleaching when compared to its control. It also had the largest decrease in fluorescence following photobleaching. Fluorescence was measured at (ex/em = 585nm/610nm).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 111
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1491021 short</partinfo>
 Codon optimized version of the monomeric superoxide generator Super Nova (BBa_K1491017) with a constitutive promoter (BBa_J23100) and a ribosome binding site (BBa_B0034). Super Nova is the monomeric form of Killer Red (BBa_K1184000).
+<hr>
+<p>Of the three superoxide generators, Supernova had the biggest decrease in viability following photobleaching when compared to its control. It also had the largest decrease in fluorescence following photobleaching. Fluorescence was measured at (ex/em = 585nm/610nm).</p>
+https://static.igem.org/mediawiki/2014/thumb/b/b0/Colonies.jpg/800px-Colonies.jpg
+<hr>
+https://static.igem.org/mediawiki/2014/thumb/e/e0/RFU.jpg/736px-RFU.jpg
 <!-- Add more about the biology of this part here