Difference between revisions of "Part:BBa K1520009"
(One intermediate revision by the same user not shown) | |||
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1520009 short</partinfo> | <partinfo>BBa_K1520009 short</partinfo> | ||
Plac-rbs-luxI-Ter. | Plac-rbs-luxI-Ter. | ||
− | It produces luxI when there is | + | |
+ | It produces luxI when there is less lacI or most of lacI is occupied by IPTG. | ||
+ | |||
+ | [[File:k1520009.jpg]] | ||
+ | From left to right, Marker(DL2000), Plac, Plac-rbs-luxI, Plac-rbs-luxI-T. The substances of electrophoresis are made from PCR of plasmid with VF2 and VR as their primers. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 01:06, 18 October 2014
Plac-rbs-luxI-Ter
Plac-rbs-luxI-Ter.
It produces luxI when there is less lacI or most of lacI is occupied by IPTG.
From left to right, Marker(DL2000), Plac, Plac-rbs-luxI, Plac-rbs-luxI-T. The substances of electrophoresis are made from PCR of plasmid with VF2 and VR as their primers.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 863
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]