Difference between revisions of "Part:BBa K1520017"
 
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Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter-Plac-rbs-rfp-Ter.
 
Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter-Plac-rbs-rfp-Ter.
  
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It is a high level detection plasmid. It produces rfp when AHL is below a certain level.  
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It is a high level detection plasmid. It produces rfp when AHL is below a certain level. The following picture shows that without AHL, the bacteria with the plasmid can express rfp.
  
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[[File:withoutAHL.jpg]]
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[[File:k1520017.jpg]]
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From left to right, Marker(1Kb), Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter, Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter-Plac-rbs-rfp-Ter
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The substances of electrophoresis are made from PCR of plasmids with VF2 and VR as their primers.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 00:52, 18 October 2014

Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter-Plac-rbs-rfp-Ter

Plasmid1 in detector: Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter-Plac-rbs-rfp-Ter.

It is a high level detection plasmid. It produces rfp when AHL is below a certain level. The following picture shows that without AHL, the bacteria with the plasmid can express rfp.

WithoutAHL.jpg

K1520017.jpg

From left to right, Marker(1Kb), Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter, Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter-Plac-rbs-rfp-Ter

The substances of electrophoresis are made from PCR of plasmids with VF2 and VR as their primers.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2215
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985