Difference between revisions of "Part:BBa R0080"

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https://static.igem.org/mediawiki/2014/f/f9/NUDT_CHINA_2014_K1532008.png
 
https://static.igem.org/mediawiki/2014/f/f9/NUDT_CHINA_2014_K1532008.png
  
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Fluorometric measurement were processed with Fluoroskan(R) Ascent FL from ThermoTM using the filters of 485nm and 538nm. The E.coli to be tested was cultured in liquid LB media containing 35μg/mL chloramphenicol to OD1.6, than split into 96 well plates (from ThermoTM) by 150μL/well. The plate was then put into the Fluoroskan(R) Ascent FL and cultured in 25℃ and background shake of 90rpms. The Fluorometric measurement was processed with the interval time of 10 minutes.
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The layout of the plate is as follows:
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https://static.igem.org/mediawiki/2014/5/50/NUDT_CHINA_R0080_CON1.jpg
  
  

Revision as of 00:46, 18 October 2014

Promoter (AraC regulated)

AraC operator, truncated to include araO1, araI1, araI2, c-amp1, and c-amp2 sites. This operator should *activate* transcription in the presence of AraC or the simple sugar [http://openwetware.org/wiki/Arabinose arabinose]

b/c the operator lacks the araO2 site, there should not be araC-mediated repression.


Usage and Biology

araC binding region from E.coli


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 103
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Contribution: iGEM14_NUDT_CHINA

Group: iGEM14_NUDT_CHINA/ Author: Xinyuan Qiu

Summary: We tested expression of this part in E.coli, and corrected the founction description of this part.

Methods and Practices

We used the circuit of BBa_K1532008 to test the function of this part, the sequence and features of the part BBa_K1532008 is as follows: NUDT_CHINA_2014_K1532008.png

Fluorometric measurement were processed with Fluoroskan(R) Ascent FL from ThermoTM using the filters of 485nm and 538nm. The E.coli to be tested was cultured in liquid LB media containing 35μg/mL chloramphenicol to OD1.6, than split into 96 well plates (from ThermoTM) by 150μL/well. The plate was then put into the Fluoroskan(R) Ascent FL and cultured in 25℃ and background shake of 90rpms. The Fluorometric measurement was processed with the interval time of 10 minutes.

The layout of the plate is as follows: NUDT_CHINA_R0080_CON1.jpg