Difference between revisions of "Part:BBa K1520004"
 
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[[File:K1520004.jpg]]
 
[[File:K1520004.jpg]]
  
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Primers used in PCR are VF2 and VR
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From left to right M：Marker（DL2000）, a: Prlux, Prlux-rbs-lacI, Prlux-rbs-lacI-T. The substances of electrophoresis are made from PCR of plasmids with VF2 and VR as their primers.
  
 
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Latest revision as of 00:45, 18 October 2014

Prlux-rbs-lacI-Ter

Prlux-rbs-lacI-Ter. It worked out an expression of lacI in the presence of luxR and AHL.

K1520004.jpg

From left to right M：Marker（DL2000）, a: Prlux, Prlux-rbs-lacI, Prlux-rbs-lacI-T. The substances of electrophoresis are made from PCR of plasmids with VF2 and VR as their primers.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1228
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]