Difference between revisions of "Part:BBa K1497004"
 
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The Chalcone Synthase catalyzes the reaction from 4-Coumaroyl-CoA to Naringenin Chalcone. It is one of the essential enzymes in the anthocyanin pathway. The CHS needs a high amount of Malonyl-CoA to constantly perform the reaction. So it has to be noted, that if used in <i> E. coli </i> the source of Malonyl-CoA is limited.
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The Chalcone Synthase catalyzes the reaction from 4-Coumaroyl-CoA to naringenin chalcone. It is one of the essential enzymes in the anthocyanin pathway. The CHS needs a high amount of Malonyl-CoA to constantly perform the reaction. So it has to be noted, that if used in <i> E. coli </i> the source of Malonyl-CoA is limited.
  
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The iGEM Team TU Darmstadt used the CHS and verified the function in their naringenin operon (<a href="/Part:BBa_K1497007">BBa_K1497007</a>).
 
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       <p class="MsoCaption" align="text-align:justify"><span lang="EN-US"><b>Figure 1</b></span></a><span lang="EN-US">
 
       <p class="MsoCaption" align="text-align:justify"><span lang="EN-US"><b>Figure 1</b></span></a><span lang="EN-US">
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Reaction of the CHS. 4-coumaroyl-CoA reacts together with 3 Malonyl-CoA to one Naringenin Calcone.</span></p>
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Reaction of the CHS. 4-coumaroyl-CoA reacts together with 3 Malonyl-CoA to one naringenin chalcone.</span></p>
 
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For using the CHI in our Naringenin Operon (K1497007) we added the strong RBS B0034.
 
  
 
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Latest revision as of 00:24, 18 October 2014

B0034-CHS - Chalcone Synthase from Gerbera with strong RBS

The Chalcone Synthase catalyzes the reaction from 4-Coumaroyl-CoA to naringenin chalcone. It is one of the essential enzymes in the anthocyanin pathway. The CHS needs a high amount of Malonyl-CoA to constantly perform the reaction. So it has to be noted, that if used in E. coli the source of Malonyl-CoA is limited.

The iGEM Team TU Darmstadt used the CHS and verified the function in their naringenin operon (BBa_K1497007).


Figure 1 Reaction of the CHS. 4-coumaroyl-CoA reacts together with 3 Malonyl-CoA to one naringenin chalcone.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 543
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 552
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 523
    Illegal NgoMIV site found at 1099
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 472