Difference between revisions of "Part:BBa K1497001"

Revision as of 00:20, 18 October 2014

CHS - Chalcone Synthase from Gerbera

The Chalcone Synthase catalyzes the reaction from 4-Coumaroyl-CoA to naringenin chalcone. It is one of the essential enzymes in the anthocyanin pathway. The CHS needs a high amount of Malonyl-CoA to constantly perform the reaction. So it has to be noted, that if used in E. coli the source of Malonyl-CoA is limited.

Figure 1 Reaction of the CHS. 4-coumaroyl-CoA reacts together with 3 Malonyl-CoA to one naringenin chalcone.

Functional Parameters

The iGEM Team TU Darmstadt used the CHS and verified the function in their naringenin operon (BBa_K1497007).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 525
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 534
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 505
Illegal NgoMIV site found at 1081
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 454

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 <td style="padding: 0cm 5.4pt; vertical-align: top; width: 306.7pt; height: 214.9pt;">
-The Chalcone Synthase catalyzes the reaction from 4-Coumaroyl-CoA to Naringenin Chalcone. It is one of the essential enzymes in the anthocyanin pathway. The CHS needs a high amount of Malonyl-CoA to constantly perform the reaction. So it has to be noted, that if used in <i> E. coli </i> the source of Malonyl-CoA is limited.
+The Chalcone Synthase catalyzes the reaction from 4-Coumaroyl-CoA to naringenin chalcone. It is one of the essential enzymes in the anthocyanin pathway. The CHS needs a high amount of Malonyl-CoA to constantly perform the reaction. So it has to be noted, that if used in <i> E. coli </i> the source of Malonyl-CoA is limited.
 <br><br>
@@ Line 25: / Line 25: @@
        <br>
        <p class="MsoCaption" align="text-align:justify"><span lang="EN-US"><b>Figure 1</b></span></a><span lang="EN-US">
-Reaction of the CHS. 4-coumaroyl-CoA reacts together with 3 Malonyl-CoA to one Naringenin Calcone.</span></p>
+Reaction of the CHS. 4-coumaroyl-CoA reacts together with 3 Malonyl-CoA to one naringenin chalcone.</span></p>
        </td>
@@ Line 37: / Line 37: @@
 ===Functional Parameters===
-The iGEM Team TU Darmstadt used the CHS and verified the function in their Naringenin Operon (<html><a href="/Part:BBa_K1497007">BBa_K1497007</a></html>).
+The iGEM Team TU Darmstadt used the CHS and verified the function in their naringenin operon (<html><a href="/Part:BBa_K1497007">BBa_K1497007</a></html>).