Difference between revisions of "Part:BBa R0080"

(→‎Contribution: iGEM14_NUDT_CHINA)
(→‎Contribution: iGEM14_NUDT_CHINA)
Line 24: Line 24:
  
 
===Methods and Practices===
 
===Methods and Practices===
−
 
+
We used the circuit of BBa_K1532008 to test the function of this part, the sequence and features of the part BBa_K1532008 is as follows:
 +
https://static.igem.org/mediawiki/2014/f/f9/NUDT_CHINA_2014_K1532008.png
  
  

Revision as of 00:10, 18 October 2014

Promoter (AraC regulated)

AraC operator, truncated to include araO1, araI1, araI2, c-amp1, and c-amp2 sites. This operator should *activate* transcription in the presence of AraC or the simple sugar [http://openwetware.org/wiki/Arabinose arabinose]

b/c the operator lacks the araO2 site, there should not be araC-mediated repression.


Usage and Biology

araC binding region from E.coli


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 103
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Contribution: iGEM14_NUDT_CHINA

Group: iGEM14_NUDT_CHINA/ Author: Xinyuan Qiu

Summary: We tested expression of this part in E.coli, and corrected the founction description of this part.

Methods and Practices

We used the circuit of BBa_K1532008 to test the function of this part, the sequence and features of the part BBa_K1532008 is as follows: NUDT_CHINA_2014_K1532008.png