Difference between revisions of "Part:BBa K1491017"
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<partinfo>BBa_K1491017 short</partinfo> | <partinfo>BBa_K1491017 short</partinfo> | ||
Supernova is the monomeric version of KillerRed (BBa_K1184000). It has been codon optimized for E. coli. | Supernova is the monomeric version of KillerRed (BBa_K1184000). It has been codon optimized for E. coli. | ||
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| + | <p>Of the three superoxide generators, Supernova had the biggest decrease in viability following photobleaching when compared to its control. It also had the largest decrease in fluorescence following photobleaching. Fluorescence was measured at (ex/em = 585nm/610nm).</p> | ||
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| + | https://static.igem.org/mediawiki/2014/thumb/b/b0/Colonies.jpg/800px-Colonies.jpg | ||
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| + | https://static.igem.org/mediawiki/2014/thumb/e/e0/RFU.jpg/736px-RFU.jpg | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Revision as of 23:49, 17 October 2014
Codon Optimized Supernova
Supernova is the monomeric version of KillerRed (BBa_K1184000). It has been codon optimized for E. coli.
Of the three superoxide generators, Supernova had the biggest decrease in viability following photobleaching when compared to its control. It also had the largest decrease in fluorescence following photobleaching. Fluorescence was measured at (ex/em = 585nm/610nm).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 50
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]