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===Applications of BBa_K1497007===
 
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  The iGEM Team TU Darmstadt 2014 created the naringenin biosynthesis operons under the control of the T7 promoter <a href="/Part:BBa_ I712074">BBa_I712074</a> and the strong constitutive promoter <a href="/Part:BBa_J23100">BBa_J23100</a>, respectively . They measured the naringenin production after a 16 h incubation time with the naringenin biosensor <a href="/Part:BBa_K1497020">BBa_K1497020</a>. <br><br>The cell pellets from E. coli BL21(DE3) – pSB1C3-fdeR-gfp with and without T7-naringenin operon (<a href="/Part:BBa_K1497017">BBa_K1497017</a>) are shown in figure 3. Only in the cell pellet with <a href="/Part:BBa_K1497017">BBa_K1497017</a> exhibited GFP fluorescence. <br><br>The Darmstadt team was also able to measure the GFP fluorescence quantitatively and to calculate with the help of a calibration curve for the naringenin sensor the production yield of both operons (Figure 4). For <a href="/Part:BBa_K1497017">BBa_K1497017</a> was 3 µM naringenin calculated and for the operon with the constitutive promoter <a href="/Part:BBa_J23100">BBa_J23100</a> (<a href="/Part:BBa_K1497016">BBa_K1497016</a>) was 1.9 µM naringenin calculated.</td>
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<font color="#FFFFFF">iGEM TU Darmstadt 2014 :)</font><br>
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       <p class="MsoCaption" align="text-align:justify"><span lang="EN-US"><b>Figure 2</b></span></a><span lang="EN-US">
 
       <p class="MsoCaption" align="text-align:justify"><span lang="EN-US"><b>Figure 2</b></span></a><span lang="EN-US">
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  E. coli Top10 with different Naringenin biosensors. Left: On agar plate without naringenin no colour is visible. Middle: On agar plate with 100 µM naringenin colour is visible, except of negative sample <a href="/Part:BBa_K1497019">BBa_K1497019</a> without fluorphor. Right: On agar plate with 100 µM Naringenin under UV light. The fluorescence of GFP, CFP and mKate is visible. <br></span></p>
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  Cell pellets with and without T7-Naringenin operon from <i>E. coli</i> BL21(DE3)-pSB1C3-<i>fdeR-gfp</i>. By using ultraviolet light the pellet containing the naringenin operon shows a GFP fluorescence.<br></span></p>
 
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      <p class="MsoCaption" align="text-align:justify"><span lang="EN-US"><b>Figure 1</b></span></a><span lang="EN-US">
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Fluorescence of cells with and without the T7-naringenin operon <a href="/Part:BBa_K1497017">BBa_K1497017</a>  from <i>E. coli</i> BL21(DE3)-pSB1C3-<i>fdeR-gfp</i> and J23100-naringenin operon (<a href="/Part:BBa_K1497016">BBa_K1497016</a>) from <i>E. coli</i> Top10-pSB1C3-<i>fdeR-gfp</i>, respectively. <i>E. coli</i> BL21(DE3)-pSB1C3-<i>fdeR-gfp</i> without T7-naringenin operon showed no detectable fluorescence. Only in the cells with the functional operon is the GFP fluorescence measurable. The estimated yields are 3 µM for <a href="/Part:BBa_K1497017">BBa_K1497017</a> and 1,9 µM for <a href="/Part:BBa_K1497016">BBa_K1497016</a>. <br></span></p>
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===User Reviews===
 
===User Reviews===
 
<!-- DON'T DELETE --><partinfo>BBa_K1497007 StartReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K1497007 StartReviews</partinfo>

Latest revision as of 23:01, 17 October 2014

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1497007

The iGEM Team TU Darmstadt 2014 created the naringenin biosynthesis operons under the control of the T7 promoter BBa_I712074 and the strong constitutive promoter BBa_J23100, respectively . They measured the naringenin production after a 16 h incubation time with the naringenin biosensor BBa_K1497020.

The cell pellets from E. coli BL21(DE3) – pSB1C3-fdeR-gfp with and without T7-naringenin operon (BBa_K1497017) are shown in figure 3. Only in the cell pellet with BBa_K1497017 exhibited GFP fluorescence.

The Darmstadt team was also able to measure the GFP fluorescence quantitatively and to calculate with the help of a calibration curve for the naringenin sensor the production yield of both operons (Figure 4). For BBa_K1497017 was 3 µM naringenin calculated and for the operon with the constitutive promoter BBa_J23100 (BBa_K1497016) was 1.9 µM naringenin calculated.		 iGEM TU Darmstadt 2014 :)

Figure 2 Cell pellets with and without T7-Naringenin operon from E. coli BL21(DE3)-pSB1C3-fdeR-gfp. By using ultraviolet light the pellet containing the naringenin operon shows a GFP fluorescence.

Figure 1 Fluorescence of cells with and without the T7-naringenin operon BBa_K1497017 from E. coli BL21(DE3)-pSB1C3-fdeR-gfp and J23100-naringenin operon (BBa_K1497016) from E. coli Top10-pSB1C3-fdeR-gfp, respectively. E. coli BL21(DE3)-pSB1C3-fdeR-gfp without T7-naringenin operon showed no detectable fluorescence. Only in the cells with the functional operon is the GFP fluorescence measurable. The estimated yields are 3 µM for BBa_K1497017 and 1,9 µM for BBa_K1497016.

User Reviews

UNIQ3bcd5a3910b2c726-partinfo-00000002-QINU UNIQ3bcd5a3910b2c726-partinfo-00000003-QINU