Difference between revisions of "Part:BBa K1486001"
 
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The part contains CpxR with an upstream arabinose-inducible promoter. This part is the starting point of our work with bacteria. It will be fused to sfGFP (BBa_K1486002, BBa_K1486005, BBa_K1486040 & BBa_K1486041) and to IFP (BBa_K1486008 to BBa_K1486015 & BBa_K1486056).
 
The part contains CpxR with an upstream arabinose-inducible promoter. This part is the starting point of our work with bacteria. It will be fused to sfGFP (BBa_K1486002, BBa_K1486005, BBa_K1486040 & BBa_K1486041) and to IFP (BBa_K1486008 to BBa_K1486015 & BBa_K1486056).
  
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===Usage and Biology===
 
===Usage and Biology===
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CpxR is a Transcription Factor which dimerizes upon activation. Its activation and inactivation is mediated by CpxA, a transmembrane histidine kinase or phosphatase when active or inactive respectively. CpxA becomes activated in response to periplasmic stress, such as outer membrane deformation, pH, salt concentration, temperature... Learn more about this pathway here: http://2014.igem.org/Team:EPF_Lausanne/Envelope_stress_responsive_bacteria
  
 
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Latest revision as of 22:55, 17 October 2014

Arabinose promoter + CpxR

The part contains CpxR with an upstream arabinose-inducible promoter. This part is the starting point of our work with bacteria. It will be fused to sfGFP (BBa_K1486002, BBa_K1486005, BBa_K1486040 & BBa_K1486041) and to IFP (BBa_K1486008 to BBa_K1486015 & BBa_K1486056).


Usage and Biology

CpxR is a Transcription Factor which dimerizes upon activation. Its activation and inactivation is mediated by CpxA, a transmembrane histidine kinase or phosphatase when active or inactive respectively. CpxA becomes activated in response to periplasmic stress, such as outer membrane deformation, pH, salt concentration, temperature... Learn more about this pathway here: http://2014.igem.org/Team:EPF_Lausanne/Envelope_stress_responsive_bacteria

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 1283
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1694
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961