Difference between revisions of "Part:BBa K1431812:Design"
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Revision as of 22:36, 17 October 2014
amilCP, blue chromoprotein reporter system (Strong Promoter, Strong RBS)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This Biobrick is constructed through 3 steps:
- Do double enzyme digestion (XbaI & PstI for BBa_K592009, SpeI & PstI for BBa_B0034) and do gel extraction, ligation, transformation, picking up single colonies, LB broth incubation, plasmid extraction and gel electrophoresis verification.
- Do double enzyme digestion (XbaI & PstI for Step 1 product, SpeI & PstI for J23100) and do gel extraction, ligation, transformation, picking up single colonies, LB broth incubation, plasmid extraction and gel electrophoresis verification.
- Do double enzyme digestion (EcoRI-HF & SpeI for Step 2 product, EcoRI-HF & XbaI for B0015) and do gel extraction, ligation, transformation, picking up single colonies, LB broth incubation, plasmid extraction and gel electrophoresis verification.
Source
The following list is the Biobricks we've used and their detailed design information.
| Part | Type | Discription | Backbone | Location on the plate |
|---|---|---|---|---|
| BBa_J23100 | Promoter | Strong Promoter | BBa_J61002 (Amp) | 17D 2014 Kit Plate 4 |
| BBa_J23106 | Promoter | Weak Promoter | BBa_J61002 (Amp) | 17P 2014 Kit Plate 4 |
| BBa_B0031 | RBS | Weak RBS | pSB1A2 | 1H 2014 Kit Plate 4 |
| BBa_B0034 | RBS | Strong RBS | pSB1A2 | 1N 2014 Kit Plate 4 |
| BBa_B0015 | Terminator | Strong terminator (B0010+B0012) | pSB1C3 | 3F 2014 Kit Plate 3 |
| BBa_K592009 | Chromoprotein | amilCP, blue chromoprotein | pSB1C3 | 19E 2014 Kit Plate 1 |
| BBa_K592011 | Chromoprotein | cjBlue, green chromoprotein | pSB1C3 | 2I 2014 Kit Plate 4 |
| BBa_K1033916 | Chromoprotein | amajLime, yellow-green chromoprote | pSB1C3 | 6M 2014 Kit Plate 4 |