Difference between revisions of "Part:BBa K1431812:Design"
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| Line 16: | Line 16: | ||
! Discription | ! Discription | ||
! Backbone | ! Backbone | ||
| − | ! Location on the | + | ! Location on the plate |
| − | + | ||
|- | |- | ||
| BBa_J23100 | | BBa_J23100 | ||
| Promoter | | Promoter | ||
| Strong Promoter | | Strong Promoter | ||
| − | + | | BBa_J61002 (Amp) | |
| − | | BBa_J61002 | + | | 17D 2014 Kit Plate 4 |
| − | + | ||
| − | | 17D 2014 Kit | + | |
| − | + | ||
|- | |- | ||
| BBa_J23106 | | BBa_J23106 | ||
| Promoter | | Promoter | ||
| Weak Promoter | | Weak Promoter | ||
| − | + | | BBa_J61002 (Amp) | |
| − | | BBa_J61002 | + | | 17P 2014 Kit Plate 4 |
| − | + | ||
| − | | 17P 2014 Kit | + | |
| − | + | ||
|- | |- | ||
| BBa_B0031 | | BBa_B0031 | ||
| RBS | | RBS | ||
| − | | Weak RBS | + | | Weak RBS |
| − | + | ||
| pSB1A2 | | pSB1A2 | ||
| − | | 1H 2014 Kit | + | | 1H 2014 Kit Plate 4 |
| − | + | ||
|- | |- | ||
| BBa_B0034 | | BBa_B0034 | ||
| Line 49: | Line 40: | ||
| Strong RBS | | Strong RBS | ||
| pSB1A2 | | pSB1A2 | ||
| − | | 1N 2014 Kit | + | | 1N 2014 Kit Plate 4 |
| − | + | ||
|- | |- | ||
| BBa_B0015 | | BBa_B0015 | ||
| Terminator | | Terminator | ||
| − | | Strong | + | | Strong terminator (B0010+B0012) |
| − | + | ||
| pSB1C3 | | pSB1C3 | ||
| 3F 2014 Kit Plate 3 | | 3F 2014 Kit Plate 3 | ||
| Line 61: | Line 50: | ||
| BBa_K592009 | | BBa_K592009 | ||
| Chromoprotein | | Chromoprotein | ||
| − | | amilCP, blue | + | | amilCP, blue chromoprotein |
| − | + | ||
| pSB1C3 | | pSB1C3 | ||
| − | | 19E 2014 Kit | + | | 19E 2014 Kit Plate 1 |
| − | + | ||
|- | |- | ||
| BBa_K592011 | | BBa_K592011 | ||
| Chromoprotein | | Chromoprotein | ||
| − | | cjBlue, green | + | | cjBlue, green chromoprotein |
| − | + | ||
| pSB1C3 | | pSB1C3 | ||
| − | | 2I 2014 Kit | + | | 2I 2014 Kit Plate 4 |
| − | + | ||
|- | |- | ||
| BBa_K1033916 | | BBa_K1033916 | ||
| Chromoprotein | | Chromoprotein | ||
| − | | amajLime, | + | | amajLime, yellow-green chromoprote |
| − | + | ||
| pSB1C3 | | pSB1C3 | ||
| − | | 6M 2014 Kit | + | | 6M 2014 Kit Plate 4 |
| − | + | ||
|} | |} | ||
===References=== | ===References=== | ||
Revision as of 22:24, 17 October 2014
amilCP, blue chromoprotein reporter system (Strong Promoter, Strong RBS)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
will be added later
Source
| Part | Type | Discription | Backbone | Location on the plate |
|---|---|---|---|---|
| BBa_J23100 | Promoter | Strong Promoter | BBa_J61002 (Amp) | 17D 2014 Kit Plate 4 |
| BBa_J23106 | Promoter | Weak Promoter | BBa_J61002 (Amp) | 17P 2014 Kit Plate 4 |
| BBa_B0031 | RBS | Weak RBS | pSB1A2 | 1H 2014 Kit Plate 4 |
| BBa_B0034 | RBS | Strong RBS | pSB1A2 | 1N 2014 Kit Plate 4 |
| BBa_B0015 | Terminator | Strong terminator (B0010+B0012) | pSB1C3 | 3F 2014 Kit Plate 3 |
| BBa_K592009 | Chromoprotein | amilCP, blue chromoprotein | pSB1C3 | 19E 2014 Kit Plate 1 |
| BBa_K592011 | Chromoprotein | cjBlue, green chromoprotein | pSB1C3 | 2I 2014 Kit Plate 4 |
| BBa_K1033916 | Chromoprotein | amajLime, yellow-green chromoprote | pSB1C3 | 6M 2014 Kit Plate 4 |