Difference between revisions of "Part:BBa K1431812:Design"

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Revision as of 22:20, 17 October 2014

amilCP, blue chromoprotein reporter system (Strong Promoter, Strong RBS)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

will be added later


Source

Part Type Discription Backbone Location on the 
 plate
BBa_J23100 Promoter Strong Promoter 
 (See the bellow figure)
BBa_J61002 
 (Amp)
17D 2014 Kit 
 Plate 4
BBa_J23106 Promoter Weak Promoter 
 (See the bellow figure)
BBa_J61002 
 (Amp)
17P 2014 Kit 
 Plate 4
BBa_B0031 RBS Weak RBS (7% 
 B0034)
pSB1A2 1H 2014 Kit 
 Plate 4
BBa_B0034 RBS Strong RBS pSB1A2 1N 2014 Kit 
 Plate 4
BBa_B0015 Terminator Strong 
 terminator (B0010+B0012), most commonly used
pSB1C3 3F 2014 Kit Plate 3
BBa_K592009 Chromoprotein amilCP, blue 
 chromoprotein
pSB1C3 19E 2014 Kit 
 Plate 1
BBa_K592011 Chromoprotein cjBlue, green 
 chromoprotein
pSB1C3 2I 2014 Kit 
 Plate 4
BBa_K1033916 Chromoprotein amajLime, 
 yellow-green chromoprote
pSB1C3 6M 2014 Kit 
 Plate 4

References