Difference between revisions of "Part:BBa K592100:Experience"

Latest revision as of 22:13, 17 October 2014

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Please enter how you used this part and how it worked out.

Applications of BBa_K592100

iGEM11_Uppsala-Sweden. BFP used as reporter. This part has been successfully used as a reporter gene for promoter characterization using flow cytometry. The excitation peak at 399 nm makes it well suited for use in flow cytometers using a 407 nm violet laser such as the FACSAria II, and the emission peak at 456 works well with a DAPI filter (band pass 450/40). The Y axis measures the promoter activity in RPU, relative promoter units, where the activity of the J23101 promoter is defined as 1 RPU.

iGEM14_Carnegie_Mellon. We characterized a set of fluorescent proteins consisting of BFP. GFP, YFP, OFP, and RFP. We calculated the signal-to-noise ratio of all the proteins in two different cell lines (MACH and Top10). BFP had the lowest signal-to-noise ratio in both cell lines. BFP was measured at (ex/em = 399nm/456nm).

User Reviews

UNIQ6ad894c3f0673a6c-partinfo-00000000-QINU UNIQ6ad894c3f0673a6c-partinfo-00000001-QINU

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 '''iGEM14_Carnegie_Mellon'''.
-We characterized a set of fluorescent proteins consisting of BFP. GFP, YFP, OFP, and RFP. We calculated the signal-to-noise ratio of all the proteins in two different cell line (MACH and Top10). BFP had the lowest signal-to-noise ratio in both cell lines. BFP was measured at ex/em = 399nm/456nm.
+We characterized a set of fluorescent proteins consisting of BFP. GFP, YFP, OFP, and RFP. We calculated the signal-to-noise ratio of all the proteins in two different cell lines (MACH and Top10). BFP had the lowest signal-to-noise ratio in both cell lines. BFP was measured at (ex/em = 399nm/456nm).
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 https://static.igem.org/mediawiki/2014/thumb/c/c1/FP_MACH.png/591px-FP_MACH.png