Difference between revisions of "Part:BBa K592100:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K592100=== | ===Applications of BBa_K592100=== | ||
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| + | [[Image:Promoter characterization.png]] | ||
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| + | '''iGEM11_Uppsala-Sweden'''. BFP used as reporter. This part has been successfully used as a reporter gene for promoter characterization using flow cytometry. The excitation peak at 399 nm makes it well suited for use in flow cytometers using a 407 nm violet laser such as the FACSAria II, and the emission peak at 456 works well with a DAPI filter (band pass 450/40). The Y axis measures the promoter activity in RPU, relative promoter units, where the activity of the J23101 promoter is defined as 1 RPU. | ||
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| + | <hr> | ||
| + | <p> | ||
| + | '''iGEM14_Carnegie_Mellon'''. | ||
| + | We characterized a set of fluorescent proteins consisting of BFP. GFP, YFP, OFP, and RFP. We calculated the signal-to-noise ratio of all the proteins in two different cell lines (MACH and Top10). BFP had the lowest signal-to-noise ratio in both cell lines. BFP was measured at (ex/em = 399nm/456nm). | ||
| + | </p> | ||
| + | https://static.igem.org/mediawiki/2014/thumb/c/c1/FP_MACH.png/591px-FP_MACH.png | ||
| + | https://static.igem.org/mediawiki/2014/thumb/9/9f/Top10_Best.jpg/591px-Top10_Best.jpg | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 22:13, 17 October 2014
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Applications of BBa_K592100
iGEM11_Uppsala-Sweden. BFP used as reporter. This part has been successfully used as a reporter gene for promoter characterization using flow cytometry. The excitation peak at 399 nm makes it well suited for use in flow cytometers using a 407 nm violet laser such as the FACSAria II, and the emission peak at 456 works well with a DAPI filter (band pass 450/40). The Y axis measures the promoter activity in RPU, relative promoter units, where the activity of the J23101 promoter is defined as 1 RPU.
iGEM14_Carnegie_Mellon. We characterized a set of fluorescent proteins consisting of BFP. GFP, YFP, OFP, and RFP. We calculated the signal-to-noise ratio of all the proteins in two different cell lines (MACH and Top10). BFP had the lowest signal-to-noise ratio in both cell lines. BFP was measured at (ex/em = 399nm/456nm).
User Reviews
UNIQ2e35c96578cc223a-partinfo-00000000-QINU UNIQ2e35c96578cc223a-partinfo-00000001-QINU