Difference between revisions of "Part:BBa K1316018"

Latest revision as of 21:58, 17 October 2014

RBS + csgB curli-forming gene

Curli are extracellular amyloids that form fibers. They are involved in adhesion, cell aggregation and biofilm formation. CsgB is one of the curli-forming proteins. More specifically, it is a subunit that stands at the beginning of each curli fiber anchored to the bacterial membrane.

Characterisation

This part was made to construct BioBricks BBa_K1316013 and BBa_K1316014. For more information about the characterisation of this construct visit the pages of these BioBricks or the Conductive curli module characterisation on our wiki page (http://2014.igem.org/Team:TU_Delft-Leiden/Project/Life_science/curli/characterisation)!

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 516
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1316018 short</partinfo>
-This part is a tightly regulated promoter that allows for high gene expression under the presence of L-rhamnose. At the same time, it shows good repression levels when L-rhamnose is not present, as well as with the presence of D-glucose. For more information read the description of part BBa_K914003, where we obtained the promoter from.
+Curli are extracellular amyloids that form fibers. They are involved in adhesion, cell aggregation and biofilm formation. CsgB is one of the curli-forming proteins. More specifically, it is a subunit that stands at the beginning of each curli fiber anchored to the bacterial membrane.
+<h3> Characterisation </h3>
+This part was made to construct BioBricks BBa_K1316013 and BBa_K1316014. For more information about the characterisation of this construct visit the pages of these BioBricks or the Conductive curli module characterisation on our wiki page (http://2014.igem.org/Team:TU_Delft-Leiden/Project/Life_science/curli/characterisation)!
 <!-- Add more about the biology of this part here