Difference between revisions of "Part:BBa K1316004"

Latest revision as of 21:31, 17 October 2014

ybiJ promoter

Promoter of the ybiJ gene, which is activated by the presence of several nitrogen-based compounds such as 2,4,6-TNT, 2,4-DNT and 1,3-DNB.[1]

Characterisation

The functionality of the ybiJ promoter is proven by the production of mKate2 in response to 2,4-DNT by the BioBrick BBa_K1316007. This promoter, besides, seems to be more sensitive than the yqjF promoter regarding its induction with 2,4-DNT.

References

[1] S. Yagur-Kroll, S. Belkin et al., “Escherichia Coli bioreporters for the detection of 2,4-dinitrotoluene and 2,4,6-trinitrotoluene”, Appl. Microbiol. Biotechnol. 98, 885-895, 2014.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 381
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1316004 short</partinfo>
-Promoter of the ybiJ gene, which is activated by the presence of several nitrogen-based compounds such as 2,4,6-TNT, 2,4-DNT and 1,3-DNB.
+Promoter of the ''ybiJ'' gene, which is activated by the presence of several nitrogen-based compounds such as 2,4,6-TNT, 2,4-DNT and 1,3-DNB.[1]
 <h3>Characterisation</h3>
-The functionality of the ''ybiJ'' promoter is proven by the production of mKate2 in response to 2,4-DNT by the BioBrick BBa_K1316005. Its response in front of 2,4-DNT, nevertheless, is much lower than the one of the yqjF promoter (BioBrick BBa_K1316003) as it can be seen on the characterisation of BioBrick BBa_K1316005 (https://parts.igem.org/Part:BBa_K1316005).
+The functionality of the ''ybiJ'' promoter is proven by the production of mKate2 in response to 2,4-DNT by the BioBrick BBa_K1316007. This promoter, besides, seems to be more sensitive than the yqjF promoter regarding its induction with 2,4-DNT.
+<h3> References </h3>
+<p>[1] S. Yagur-Kroll, S. Belkin <i>et al.</i>, “<i>Escherichia Coli</i> bioreporters for the detection of 2,4-dinitrotoluene and 2,4,6-trinitrotoluene”, Appl. Microbiol. Biotechnol. 98, 885-895, 2014. </p>