Difference between revisions of "Part:BBa K1545001"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1545001 short</partinfo> | <partinfo>BBa_K1545001 short</partinfo> | ||
| − | Array of 6 binding sites for gRNAs matching CCATCTAATTCAACAAGAATTGG + 7 bp spacer | + | Array of 6 binding sites for gRNAs matching CCATCTAATTCAACAAGAATTGG + 7 bp spacer. |
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| + | Use this as a decoy to change degree of repression by dCas9 of GFP (BBa_K608012). Bacteria harboring dCas9 targeting this site have less repression of GFP in proportion to the copies of decoy present as shown below: | ||
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| + | [[File:BiggerDecoyReflow.jpg]] | ||
| + | Addition of pSB1A2-K154501 to strains containing pSB4K5-K608012 and pdCas9 with crRNA targeting GFP1 sequence 7.5-fold relative to pSB1A2 with no decoys. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 21:19, 17 October 2014
6xGFP1 decoy
Array of 6 binding sites for gRNAs matching CCATCTAATTCAACAAGAATTGG + 7 bp spacer.
Use this as a decoy to change degree of repression by dCas9 of GFP (BBa_K608012). Bacteria harboring dCas9 targeting this site have less repression of GFP in proportion to the copies of decoy present as shown below:
Addition of pSB1A2-K154501 to strains containing pSB4K5-K608012 and pdCas9 with crRNA targeting GFP1 sequence 7.5-fold relative to pSB1A2 with no decoys. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]