Difference between revisions of "Part:BBa K1545000"
 
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<partinfo>BBa_K1545000 short</partinfo>
 
<partinfo>BBa_K1545000 short</partinfo>
  
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Array of 1 binding site for gRNAs matching CCATCTAATTCAACAAGAATTGG + 7 bp spacer
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Array of 1 binding site for gRNAs matching CCATCTAATTCAACAAGAATTGG + 7 bp spacer.
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Use this as a decoy to change degree of repression by dCas9 of GFP (BBa_K608012). Bacteria harboring dCas9 targeting this site have less repression of GFP in proportion to the copies of decoy present as shown below:
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[[File:BiggerDecoyReflow.jpg]]
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Addition of pSB1A2-K154500 to strains containing pSB4K5-K608012 and pdCas9 with crRNA targeting GFP1 sequence 1.7-fold relative to pSB1A2 with no decoys.
  
 
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===Usage and Biology===
 
===Usage and Biology===
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Use this part as a decoy binding site for dCas9 targeting BBa_K608012 at CCATCTAATTCAACAAGAATTGG.
 
  
 
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Latest revision as of 21:18, 17 October 2014

1xGFP1 decoy

Array of 1 binding site for gRNAs matching CCATCTAATTCAACAAGAATTGG + 7 bp spacer.

Use this as a decoy to change degree of repression by dCas9 of GFP (BBa_K608012). Bacteria harboring dCas9 targeting this site have less repression of GFP in proportion to the copies of decoy present as shown below:

BiggerDecoyReflow.jpg

Addition of pSB1A2-K154500 to strains containing pSB4K5-K608012 and pdCas9 with crRNA targeting GFP1 sequence 1.7-fold relative to pSB1A2 with no decoys.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]