Difference between revisions of "Part:BBa K1497017:Experience"

 
(→‎Applications of BBa_K1497017)
 
Line 5: Line 5:
  
 
===Applications of BBa_K1497017===
 
===Applications of BBa_K1497017===
 +
 +
 +
<html>
 +
<div align="right">
 +
<table class="MsoTableGrid"
 +
style="border: medium none ; border-collapse: collapse; text-align: left; margin-left: auto; margin-right: auto;"
 +
border="0" cellpadding="0" cellspacing="0">
 +
  <tbody>
 +
    <tr style="height: 214.9pt;">
 +
   
 +
<td style="padding: 0cm 5.4pt; width: 336.7pt; height: 214.9pt;">
 +
  The iGEM Team TU Darmstadt 2014 created the naringenin biosynthesis operons under the control of the T7 promoter <a href="/Part:BBa_ I712074">BBa_I712074</a> and the strong constitutive promoter <a href="/Part:BBa_J23100">BBa_J23100</a>, respectively . They measured the naringenin production after a 16 h incubation time with the naringenin biosensor <a href="/Part:BBa_K1497020">BBa_K1497020</a>. <br><br>The cell pellets from E. coli BL21(DE3) – pSB1C3-fdeR-gfp with and without T7-naringenin operon (<a href="/Part:BBa_K1497017">BBa_K1497017</a>) are shown in figure 3. Only in the cell pellet with <a href="/Part:BBa_K1497017">BBa_K1497017</a> exhibited GFP fluorescence. <br><br>The Darmstadt team was also able to measure the GFP fluorescence quantitatively and to calculate with the help of a calibration curve for the naringenin sensor the production yield of both operons (Figure 4). For <a href="/Part:BBa_K1497017">BBa_K1497017</a> was 3 µM naringenin calculated and for the operon with the constitutive promoter <a href="/Part:BBa_J23100">BBa_J23100</a> (<a href="/Part:BBa_K1497016">BBa_K1497016</a>) was 1.9 µM naringenin calculated.</td>
 +
<td>
 +
 +
<font color="#FFFFFF">iGEM TU Darmstadt 2014 :)</font><br>
 +
</td><td
 +
style="padding: 0cm 5.4pt; vertical-align: top; width: 250.7pt; height: 170.9pt;">
 +
      <img style="width: 350px; height: 38,4px;" alt=""
 +
src="https://static.igem.org/mediawiki/parts/e/e6/Naringeninoperont7coli.png"></p>
 +
     
 +
      <p class="MsoCaption" align="text-align:justify"><span lang="EN-US"><b>Figure 2</b></span></a><span lang="EN-US">
 +
Cell pellets with and without T7-Naringenin operon from <i>E. coli</i> BL21(DE3)-pSB1C3-<i>fdeR-gfp</i>. By using ultraviolet light the pellet containing the naringenin operon shows a GFP fluorescence.<br></span></p>
 +
      </td>
 +
 +
    </tr>
 +
<tbody>
 +
</table>
 +
</div>
 +
</html>
 +
 +
<html>
 +
 +
<div align="right">
 +
<table class="MsoTableGrid"
 +
style="border: medium none ; border-collapse: collapse; text-align: left; margin-left: auto; margin-right: auto;"
 +
border="0" cellpadding="0" cellspacing="0">
 +
  <tbody>
 +
    <tr style="height: 214.9pt;">
 +
   
 +
 +
<td
 +
style="padding: 0cm 5.4pt; vertical-align: top; width: 250.7pt; height: 170.9pt;">
 +
      <img style="width: 750px; height: 75,4px;" alt=""
 +
src="https://static.igem.org/mediawiki/parts/b/b5/NaringenBALK.png"></p>
 +
     
 +
      <p class="MsoCaption" align="text-align:justify"><span lang="EN-US"><b>Figure 1</b></span></a><span lang="EN-US">
 +
Fluorescence of cells with and without the T7-naringenin operon <a href="/Part:BBa_K1497017">BBa_K1497017</a>  from <i>E. coli</i> BL21(DE3)-pSB1C3-<i>fdeR-gfp</i> and J23100-naringenin operon (<a href="/Part:BBa_K1497016">BBa_K1497016</a>) from <i>E. coli</i> Top10-pSB1C3-<i>fdeR-gfp</i>, respectively. <i>E. coli</i> BL21(DE3)-pSB1C3-<i>fdeR-gfp</i> without T7-naringenin operon showed no detectable fluorescence. Only in the cells with the functional operon is the GFP fluorescence measurable. The estimated yields are 3 µM this part <a href="/Part:BBa_K1497017">(BBa_K1497017)</a> and 1,9 µM for <a href="/Part:BBa_K1497016">BBa_K1497016</a>. <br></span></p>
 +
      </td>
 +
 +
    </tr>
 +
<tbody>
 +
</table>
 +
</div>
 +
</html>
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 21:13, 17 October 2014


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1497017

The iGEM Team TU Darmstadt 2014 created the naringenin biosynthesis operons under the control of the T7 promoter BBa_I712074 and the strong constitutive promoter BBa_J23100, respectively . They measured the naringenin production after a 16 h incubation time with the naringenin biosensor BBa_K1497020.

The cell pellets from E. coli BL21(DE3) – pSB1C3-fdeR-gfp with and without T7-naringenin operon (BBa_K1497017) are shown in figure 3. Only in the cell pellet with BBa_K1497017 exhibited GFP fluorescence.

The Darmstadt team was also able to measure the GFP fluorescence quantitatively and to calculate with the help of a calibration curve for the naringenin sensor the production yield of both operons (Figure 4). For BBa_K1497017 was 3 µM naringenin calculated and for the operon with the constitutive promoter BBa_J23100 (BBa_K1497016) was 1.9 µM naringenin calculated.		 iGEM TU Darmstadt 2014 :)

Figure 2 Cell pellets with and without T7-Naringenin operon from E. coli BL21(DE3)-pSB1C3-fdeR-gfp. By using ultraviolet light the pellet containing the naringenin operon shows a GFP fluorescence.

Figure 1 Fluorescence of cells with and without the T7-naringenin operon BBa_K1497017 from E. coli BL21(DE3)-pSB1C3-fdeR-gfp and J23100-naringenin operon (BBa_K1497016) from E. coli Top10-pSB1C3-fdeR-gfp, respectively. E. coli BL21(DE3)-pSB1C3-fdeR-gfp without T7-naringenin operon showed no detectable fluorescence. Only in the cells with the functional operon is the GFP fluorescence measurable. The estimated yields are 3 µM this part (BBa_K1497017) and 1,9 µM for BBa_K1497016.

User Reviews

UNIQd9db32b0ed792d7d-partinfo-00000002-QINU UNIQd9db32b0ed792d7d-partinfo-00000003-QINU