Difference between revisions of "Part:BBa K1453408"
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Revision as of 21:05, 17 October 2014
pykF-TAL1-His Tag
This part is used in our application test free in the cytoplasm. See more details please view iGEM14_SJTU_BioX_Shanghai.
This part is based on the BBa_K1453406 or BBa_K1453407 and the recognition sequence is T-TCGATATCAAGC-T. Therefore, the TAL-Protein DiRepeats protein we need are BBa_K747013, BBa_K747024, BBa_K747044, BBa_K747061, BBa_K747064 and BBa_K747089. The enzyme we used here is the pyruvate kinase (EC:2.7.1.40) or pykF.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1312
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1312
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1027
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1312
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1312
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1933
Illegal BsaI.rc site found at 2545