Difference between revisions of "Part:BBa K1453405"
FlyFreedom (Talk | contribs) |
FlyFreedom (Talk | contribs) |
||
Line 2: | Line 2: | ||
<partinfo>BBa_K1453405 short</partinfo> | <partinfo>BBa_K1453405 short</partinfo> | ||
− | This part is used in our application test. See more details please view iGEM14_SJTU_BioX_Shanghai. | + | This part is used in our application test on the membrane. See more details please view iGEM14_SJTU_BioX_Shanghai. |
== Structure == | == Structure == |
Latest revision as of 21:02, 17 October 2014
ssDsbA-Lgt-poxB-TAL1-His Tag
This part is used in our application test on the membrane. See more details please view iGEM14_SJTU_BioX_Shanghai.
Structure
Figure 1 ssDsbA-Lgt-poxB-TAL1-His Tag
This part is based on the BBa_K1453402 or BBa_K1453403 and the recognition sequence is T-TCGATATCAAGC-T. Therefore, the TAL-Protein DiRepeats protein we need are BBa_K747013, BBa_K747024, BBa_K747044, BBa_K747061, BBa_K747064 and BBa_K747089. The enzyme we used here is the pyruvate dehydrogenase (quinone) [EC:1.2.5.1] or poxB
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 862
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 483
Illegal BsaI.rc site found at 3274
Illegal BsaI.rc site found at 3886