Difference between revisions of "Part:BBa K1342002"

Latest revision as of 20:53, 17 October 2014

AspA LVA(-)

BBa_C0083 encodes aspartase with LVA tag, that tag renders protein susceptible to the action of tail-specific proteases. We removed LVA tag in BBa_C0083.
AspA is coding sequence of aspartase carries out reversible conversion of L-aspartate to fumarate and ammonia.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 72
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 964
Illegal AgeI site found at 493
Illegal AgeI site found at 1345
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 1004

TLC Assay

fig.1
Analysis of aspartic acid synthesized by E. coli JM109/BBa_K1342001 by thin-layer chromatography (TLC) Lane: 1, 2 no addition into culture (diluted 1:50 and 1:100 by synthesis medium, respectively) 3, additon of cadmium ion (250μM) 4, 5, 6 (diluted 1:50, 1:100 and 1:200 by synthesis medium, respectively)

fig.2
Estimation of aspartic acid content in spot Each intensity of spots indicating the content of aspartic acid, was estimated by Image J. .

@@ Line 6: / Line 6: @@
 [https://parts.igem.org/Part:BBa_C0083 BBa_C0083] encodes aspartase with LVA tag, that tag renders protein susceptible to the action of tail-specific proteases.
-We removes LVA tag in [https://parts.igem.org/Part:BBa_C0083 BBa_C0083].
+We removed LVA tag in [https://parts.igem.org/Part:BBa_C0083 BBa_C0083].
 <br>
 AspA is coding sequence of aspartase carries out reversible conversion of L-aspartate to fumarate and ammonia.