Difference between revisions of "Part:BBa K393000:Experience"

Latest revision as of 19:48, 17 October 2014

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K393000

The 2014 Minnesota iGEM team sought to both improve and characterize phsABC (Part:BBa_K393000), originally added by the 2010 Yale iGEM team, by incorporating a modified lac promoter that enables constitutive expression rather than IPTG induction thus making it more applicable to scale-up remediation efforts. We also improved the characterization of Yale's part by testing its application for biological precipitation of iron and cadmium in addition to their copper testing to add to the functionality of the part.

We have shown precipitation of cadium and iron using the modified BBa_K393000 which was entered as BBa_K1420006. We also added a protocol enabling the measurement of sulfides (Cline's Assay).

Link to the 2014 University of Minnesota phsABC part: https://parts.igem.org/Part:BBa_K1420006

User Reviews

UNIQb728434c0802bac7-partinfo-00000000-QINU UNIQb728434c0802bac7-partinfo-00000001-QINU

@@ Line 4: / Line 4: @@
 ===Applications of BBa_K393000===
+The 2014 Minnesota iGEM team sought to both improve and characterize phsABC (Part:BBa_K393000), originally added by the 2010 Yale iGEM team, by incorporating a modified lac promoter that enables constitutive expression rather than IPTG induction thus making it more applicable to scale-up remediation efforts. We also improved the characterization of Yale's part by testing its application for biological precipitation of iron and cadmium in addition to their copper testing to add to the functionality of the part.
+We have shown precipitation of cadium and iron using the modified BBa_K393000 which was entered as BBa_K1420006. We also added a protocol enabling the measurement of sulfides (Cline's Assay).
+Link to the 2014 University of Minnesota phsABC part: https://parts.igem.org/Part:BBa_K1420006
 ===User Reviews===
@@ Line 14: / Line 20: @@
 <I>Username</I>
 |width='60%' valign='top'|
+We never successfully ligated a promoter in front of this generator, so we were unable to test it.  It's parent plasmid (pSB74 from Bang et al AEM 2000) works well, causing Iron to precipitate in 4-5 days.
 |};
 <!-- End of the user review template -->
 <!-- DON'T DELETE --><partinfo>BBa_K393000 EndReviews</partinfo>