Difference between revisions of "Part:BBa K1462270"
Line 2: | Line 2: | ||
<partinfo>BBa_K1462270 short</partinfo> | <partinfo>BBa_K1462270 short</partinfo> | ||
− | This part | + | This part express the second enzyme ----3-hydroxybutyryl-CoA dehydrogenase. Hbd is expressed under control of GAL1 promoter and ADH1 terminator. With the help of CoxVI,the enzyme will be transported into mitochondrial matrix after being translated.Together with BBa_K1462260, BBa_K1462280, BBa_K1462290 and BBa_K1462300, this part is components of the n-butanol biosynthesis pathway. |
+ | |||
+ | [[File:Pathway.png|200px|left|frame|Figure 1. The n-butanol biosynthetic pathway. Enzymes are from these organisms: Erg10, S. cerevisiae; Hbd, Crt, AdhE2, Clostridium beijerinckii; Ccr, Streptomyces collinus.]] | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 19:41, 17 October 2014
Gal1+cox6+Hbd+ADH1
This part express the second enzyme ----3-hydroxybutyryl-CoA dehydrogenase. Hbd is expressed under control of GAL1 promoter and ADH1 terminator. With the help of CoxVI,the enzyme will be transported into mitochondrial matrix after being translated.Together with BBa_K1462260, BBa_K1462280, BBa_K1462290 and BBa_K1462300, this part is components of the n-butanol biosynthesis pathway.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 150
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1068