Difference between revisions of "Part:BBa K1462220"

Latest revision as of 19:29, 17 October 2014

Gal1+Hbd+ADH1

The n-butanol pathway in the cytoplasm is constructed as a control group to identify the effectiveness of the pathway directed to the mitochondria.Hbd,originated from Clostridium beijerinckii, expresses 3-hydroxybutyryl-CoA dehydrogenase, which converts AcAcCoA to HbCoA, the second step of the n-butanol pathway.This part is constructed in pUC57 individually at the beginning and it is assembled with Erg10(BBa_K1462210),Crt(BBa_K1462230) in plasmid 181 ,which is leucine auxotroph.

Figure 1. The n-butanol biosynthetic pathway. Enzymes are from these organisms: Erg10, S. cerevisiae; Hbd, Crt, AdhE2, Clostridium beijerinckii; Ccr, Streptomyces collinus.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 150
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 942

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 __NOTOC__
 <partinfo>BBa_K1462220 short</partinfo>
+The n-butanol pathway in the cytoplasm is constructed as a control group to identify the effectiveness of the pathway directed to the mitochondria.Hbd,originated from Clostridium beijerinckii, expresses 3-hydroxybutyryl-CoA dehydrogenase, which converts AcAcCoA to HbCoA, the second step of the n-butanol pathway.This part is constructed in pUC57 individually at the beginning and it is assembled with Erg10(BBa_K1462210),Crt(BBa_K1462230) in plasmid 181 ,which is leucine auxotroph.
+[[File:Pathway.png|200px|left|frame|Figure 1. The n-butanol biosynthetic pathway. Enzymes are from these organisms: Erg10, S. cerevisiae; Hbd, Crt, AdhE2, Clostridium beijerinckii; Ccr, Streptomyces collinus.]]
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