Difference between revisions of "Part:BBa K1415001"
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K1415002 short</partinfo>
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<partinfo>BBa_K1415001 short</partinfo>
 
<h1>'''Introduction:''' PBAN (Pheromone Biosynthesis Activating Neuropeptide)</h1>
 
<h1>'''Introduction:''' PBAN (Pheromone Biosynthesis Activating Neuropeptide)</h1>
  
  
  
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<div>[[File:MB.png|thumb|right|300px|'''Fig.1-1''' A coding gene of a <span style="font-style: italic;">Mamestra brassicae's</span> PBAN ]]</div>
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<div>[[File:BM.png|thumb|right|300px|'''Fig.1-1''' A coding gene of a <span style="font-style: italic;">Bombyx mori's</span> PBAN ]]</div>
 
<p style="font-size:120%">'''Mechanism of PBAN'''</p>
 
<p style="font-size:120%">'''Mechanism of PBAN'''</p>
 
PBAN (Pheromone Biosynthesis Activating Neuropeptide) is one kind of peptides that can activate biosynthesis of pheromones of insects we target. Once a PBAN binds with the G-protein coupled receptor on an insect’s pheromone gland, the signal send by the G-protein coupled receptor activates the kinase and phosphatase, and then kinase and phosphatase can activate enzymes that participate in the biosynthesis of insect pheromone, which will be emitted.
 
PBAN (Pheromone Biosynthesis Activating Neuropeptide) is one kind of peptides that can activate biosynthesis of pheromones of insects we target. Once a PBAN binds with the G-protein coupled receptor on an insect’s pheromone gland, the signal send by the G-protein coupled receptor activates the kinase and phosphatase, and then kinase and phosphatase can activate enzymes that participate in the biosynthesis of insect pheromone, which will be emitted.
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Together, using PBAN is totally a environmental friendly way for solving harmful insects problems with easily triggering pheromone production by contacting with its receptor.
 
Together, using PBAN is totally a environmental friendly way for solving harmful insects problems with easily triggering pheromone production by contacting with its receptor.
 
<br><br>
 
<br><br>
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<div style="font: italic bold 12px/30px Georgia, serif;">This part is a coding gene of Mamestra brassicae's PBAN.</div>
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<div style="font: italic bold 12px/30px Georgia, serif;">This part is a coding gene of Bombyx mori's PBAN.</div>
 
<br>
 
<br>
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See our expanding PBAN(Mamestra brassicae) parts collection:
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See our expanding PBAN(Bombyx mori) parts collection:
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[https://parts.igem.org/Part:BBa_K1415101 Pcons+B0034+PBAN(Mamestra brassicae)] and
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[https://parts.igem.org/Part:BBa_K1415101 Pcons+B0034+PBAN(Bombyx mori)] and
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[https://parts.igem.org/Part:BBa_K1415201 Pcons+B0034+PBAN(Mamestra brassicae)+B0034+BFP+J61048 ]
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[https://parts.igem.org/Part:BBa_K1415201 Pcons+B0034+PBAN(Bombyx mori)+B0034+BFP+J61048 ]
  
 
[[File:2014NCTUGprotein.jpg|800px|thumb|center|'''Fig.1-2''' Working mechanism of PBAN ]]
 
[[File:2014NCTUGprotein.jpg|800px|thumb|center|'''Fig.1-2''' Working mechanism of PBAN ]]
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<br><br><br>
 
<br><br><br>
  
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<h1>'''Target insect:''' Cotton bollworm (Mamestra brassicae)</h1>
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<h1>'''Target insect:''' Cotton bollworm (Bombyx mori)</h1>
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[[File:HAH-insect.png|thumb|right|900px|'''Fig.2-1''' Introduction of Mamestra brassicae]]
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[[File:HAH-insect.png|thumb|right|900px|'''Fig.2-1''' Introduction of Bombyx mori]]
  
  
 
<h1>The experiment of PBAN</h1>
 
<h1>The experiment of PBAN</h1>
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[[File:PCRMB4.png|left|thumb|900px|<p style="padding: 10px !important;">'''Fig.2-2''' The PCR result of the PBAN-MB. The DNA sequence length of PBANs are around 100～150 bp, so the PCR products should appear at 415～515 bp.</p>]]
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[[File:PCRBM4.png|left|thumb|900px|<p style="padding: 10px !important;">'''Fig.2-2''' The PCR result of the PBAN-MB. The DNA sequence length of PBANs are around 100～150 bp, so the PCR products should appear at 415～515 bp.</p>]]
 
<div style="display: block; height: 530pt;">
 
<div style="display: block; height: 530pt;">
 
After receiving the DNA sequences from the gene synthesis company, we recombined each PBAN gene to PSB1C3 backbones and conducted a PCR experiment to check the size of each of the PBANs. The DNA sequence length of the PBAN are around 100～150 bp. In this PCR experiment, the PBAN products size should be near at 415～515 bp. The '''Fig.2-2''' showed the correct size of the PBAN, and proved that we successful ligated the PBAN DNA sequence onto an ideal backbone.
 
After receiving the DNA sequences from the gene synthesis company, we recombined each PBAN gene to PSB1C3 backbones and conducted a PCR experiment to check the size of each of the PBANs. The DNA sequence length of the PBAN are around 100～150 bp. In this PCR experiment, the PBAN products size should be near at 415～515 bp. The '''Fig.2-2''' showed the correct size of the PBAN, and proved that we successful ligated the PBAN DNA sequence onto an ideal backbone.
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[[File:nctu006pcr.jpg|right|thumb|650px|'''Fig.2-3''' The plate of part-PBAN(Mamestra brassicae)]]
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[[File:nctu006pcr.jpg|right|thumb|650px|'''Fig.2-3''' The plate of part-PBAN(Bombyx mori)]]
 
</div>
 
</div>
  

Revision as of 18:59, 17 October 2014

PBAN (Bombyx mori)

Introduction: PBAN (Pheromone Biosynthesis Activating Neuropeptide)


Fig.1-1 A coding gene of a Bombyx mori's PBAN

Mechanism of PBAN

PBAN (Pheromone Biosynthesis Activating Neuropeptide) is one kind of peptides that can activate biosynthesis of pheromones of insects we target. Once a PBAN binds with the G-protein coupled receptor on an insect’s pheromone gland, the signal send by the G-protein coupled receptor activates the kinase and phosphatase, and then kinase and phosphatase can activate enzymes that participate in the biosynthesis of insect pheromone, which will be emitted.

Features of PBAN

1. Species-specific: PBAN is species-specific just like pheromones, meaning that every kind of insect produces specific PBAN that only binds with its specific receptor, resulting in the production of a particular pheromone.

2. Small and simple: The coding sequence for a PBAN is only around 100 base pairs. For E.coli, 100 base pairs is totally within its working capacity. Therefore, E.coli can be a low-cost PBAN factory. By transforming the DNA sequences for different PBAN into the E.coil, we can even gain a variety of PBANs. 　

3. Secreted directly: Because PBAN is secreted by the insect itself, the insect would not form a resistance to it compare to use pesticide.

Together, using PBAN is totally a environmental friendly way for solving harmful insects problems with easily triggering pheromone production by contacting with its receptor.

This part is a coding gene of Bombyx mori's PBAN.


See our expanding PBAN(Bombyx mori) parts collection: Pcons+B0034+PBAN(Bombyx mori) and Pcons+B0034+PBAN(Bombyx mori)+B0034+BFP+J61048

Fig.1-2 Working mechanism of PBAN
Reference:

Ada Rafaeli, Pheromone biosynthesis activating neuropeptide (PBAN): Regulatory role and mode of action, General and Comparative Endocrinology 162 (2009) 69–78.




Target insect: Cotton bollworm (Bombyx mori)

Fig.2-1 Introduction of Bombyx mori


The experiment of PBAN

Fig.2-2 The PCR result of the PBAN-MB. The DNA sequence length of PBANs are around 100～150 bp, so the PCR products should appear at 415～515 bp.

After receiving the DNA sequences from the gene synthesis company, we recombined each PBAN gene to PSB1C3 backbones and conducted a PCR experiment to check the size of each of the PBANs. The DNA sequence length of the PBAN are around 100～150 bp. In this PCR experiment, the PBAN products size should be near at 415～515 bp. The Fig.2-2 showed the correct size of the PBAN, and proved that we successful ligated the PBAN DNA sequence onto an ideal backbone.

Fig.2-3 The plate of part-PBAN(Bombyx mori)






Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 18
  • 1000
    COMPATIBLE WITH RFC[1000]