Difference between revisions of "Part:BBa K1498000:Experience"
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===Applications of BBa_K1498000=== | ===Applications of BBa_K1498000=== | ||
| − | Under the control of the constitutive promoter BBa_J23110 and following expression in ''E. coli'' BL21 the supernatant was analysed on a SDS-PAGE gel. Following coomassie staining a protein of | + | Under the control of the constitutive promoter BBa_J23110 and following expression in ''E. coli'' BL21 the supernatant was analysed on a SDS-PAGE gel. Following coomassie staining a protein of 39.4 kDa was visible, suggesting that the pre-processed protein was present. However only the processed form of the protein will exhibit activity. |
In theory BBa_K1498000 should be suitable to use in assays to degrade keratin rich structures (hair, nails, feathers). | In theory BBa_K1498000 should be suitable to use in assays to degrade keratin rich structures (hair, nails, feathers). | ||
Revision as of 18:42, 17 October 2014
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Applications of BBa_K1498000
Under the control of the constitutive promoter BBa_J23110 and following expression in E. coli BL21 the supernatant was analysed on a SDS-PAGE gel. Following coomassie staining a protein of 39.4 kDa was visible, suggesting that the pre-processed protein was present. However only the processed form of the protein will exhibit activity.
In theory BBa_K1498000 should be suitable to use in assays to degrade keratin rich structures (hair, nails, feathers).
As a protease gelatin degredation should also be observed.
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