Difference between revisions of "Part:BBa K1510010:Experience"

Latest revision as of 18:38, 17 October 2014

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Applications of BBa_K1510010

Secreting red fluorescence protein (RFP) extracellularly, it can be used to test if YebF work. Anying circuit required secreted RFP can also make application.

User Reviews

UNIQ78711517e58708f7-partinfo-00000000-QINU To test if our yebF works, we fuse RFP at the C-terminal of the protein(YebF), and test if the signal protein works by measuring the fluorescence intensity in the culture supernatant. This is our result under Multimode microplate reader: UNIQ78711517e58708f7-partinfo-00000001-QINU

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 ===Applications of BBa_K1510010===
-Secreting RFP extracellularly, it can be used to test if YebF work. Anying circuit required secreted RFP can also make application.
+Secreting red fluorescence protein (RFP) extracellularly, it can be used to test if YebF work. Anying circuit required secreted RFP can also make application.
 ===User Reviews===
 <!-- DON'T DELETE --><partinfo>BBa_K1510010 StartReviews</partinfo>
-To test if our yebF works, we fuse RFP at the C-terminal of the protein, and test if the signal protein works by examing the fluorescence intensity in the culture supernatant.
+To test if our yebF works, we fuse RFP at the C-terminal of the protein(YebF), and test if the signal protein works by measuring the fluorescence intensity in the culture supernatant.
 This is our result under Multimode microplate reader:
 [[File:NYMU yebF fluroescence intensity of RFP in supernat partial enlargement detail dataJPEG.jpg]]